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GrainGenes Sequence Report: BE498068

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Sequence	BE498068
Contig	Ta.22794.1.S1_at Ta.22794.1.S1_x_at NSFT03P2_Contig18454
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC313444
NCBI UniGene	Ta.50499
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Hypothetical LOC543011'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Clone Library	Wheat pre-anthesis spike cDNA library
Tissue	Spike before anthesis
Developmental Stage	Adult plant
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0953_H02_P03ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE0953_H02_P03, mRNA sequence.
Other Name	WHE0953_H02_P03ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA019E1X
Clone	WHE0953_H02_P03
Probe	[Wcl127ct584cn929] {SpCl-192} BE498068
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	tttttccccttccgtccgtcgccgccgctgctctagggtttcctcttccc gatccccgatccagcagcagcagcagcgagcgagcgatggcggccgcggg agacgccggcgagaagaagatgatcacgctcaagagctcggacggcgagg agttcgaggtggaggaggcggtggccatggagtcgcagacgatccgccac atgatcgaggacgactgcgccgacaacggcatcccgctccccaacgtcaa ctccaagatcctctccaaggtcatcgagtactgcaacaagcacgtccagg ccaagcccgccgacggggccgcggccgccgccggagcctcggacgccgcc gcccccaccgcccccgcggaggacctcaagaactgggacgccgagttcgt caaggtcgaccaggccaccctcttcgacctcatcctggctgccaactacc tgaacatcaaggggctgctggacctaacctgtcagactgttgccgacatg atcaagggcaagaccccggaggagatccgcaagaccttcaacatcaagaa cgactttacgcccg

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