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GrainGenes Sequence Report: BE497743

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Sequence
BE497743
Contig
Ta.28742.2.S1_x_at
NSFT03P2_Contig18462
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC333138
NCBI UniGene
Ta.36473
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_181192.1 LOS2 (Low expression of osmotically responsive genes 1); phosphopyruvate hydratase [Arabidopsis thaliana]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0956_G02_M04ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE0956_G02_M04, mRNA sequence.
Other Name
WHE0956_G02_M04ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA019E1X
Clone
WHE0956_G02_M04
Probe
[Wcl102ct509cn822]
{SpCl-193}
BE497743
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
cgccccctctcctctccgctccatctggtccgattcgattcgattcgatc
tcggcttcctctccaagccacgcgagaccagccatggcggcgacgatcca
gtccgtgaaggcccgccagatcttcgacagccgcggcaaccccaccgtcg
aggttgatgtgtgctgctcagatggaaccttcgccagggccgccgttccc
agcggtgcatcaactggtgtctatgaagctttggagctgagggacggtgg
atctgactacttgggcaagggtgtttccaaggctgttaacaatgtgaact
ccattattgcacccgctttggtcggcaaggaccctaccgctcaaactgag
ctcgacaactttatggttcagcagcttgatggaaccaagaatgagtgggg
ttggtgcaagcaaaagcttggtgctaatgcaatcctggctgtatcactag
ctgtttgcaaagctggagccagcgtcaagaagattccactgtaccagcac
attgccaaccttgctggcaacaagcaattggttttgcccgttcctgcgtt
caatgtcatcaatggtggatcccatgctggaaacaagcttgctatgcagg
agttcatgatccttcctactggagctacctcattc

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