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GrainGenes Sequence Report: NSFT03P2_Contig15196

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Sequence
BE497740
Contig
Ta.2790.1.S1_at
NSFT03P2_Contig15196
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC314997
wEST map position
BE497740
NCBI UniGene
Ta.48597
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Clone wle1n.pk0106.g11:fis, full insert mRNA sequence'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
3AL
3BL
3DL
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0956_G06_M12ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE0956_G06_M12, mRNA sequence.
Other Name
WHE0956_G06_M12ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA019E1X
Clone
WHE0956_G06_M12
Probe
WHE0956_G06_M12
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.2790.1.S1_at773WHE2AFFY
Ta.2790.1.S1_at90WHE2AFFY
DNA
ggctagctacgatccccttctgcaacccggtcgtgtctcagggcgcatcg
ccgaagccaagccctccctccctagcagcgcccggacccatcgatcccgg
cgggacgcgccgtcggcgccccaggctcaagctccatcgcgcgctcgacg
gcgagggatggatccgatggacatcgtgggcaagtccaaggaggacgtct
ccctccccaaatcaacaatgaccaagattattaaggagatgctaccgcct
gatgttcgagtagcaagagatacacaagatcttcttgttgaatgctgtgt
agagttcatcaatcttctttcttcggaatccaatgacgtgtgcagccggg
acgacaagaagactattgctcctgaacatgttattagggctctgcaggat
cttggcttcaaggagtacgttgaagaagtntatgcagcctacgaacagca
caagcttgaaactctggactctccaaaagcaaccaagttcactggtttag
agatgactgaagaagaagctgttgcggaacagcagagaatgtttgctga

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