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GrainGenes Sequence Report: BE497469

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Sequence
BE497469
Contig
Ta.28018.1.S1_at
NSFT03P2_Contig17014
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC285294
wEST map position
BE497469
NCBI UniGene
Ta.48473
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Clone wlk4.pk0006.e8:fis, full insert mRNA sequence'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
3AS
3BS
3DS
Clone Library
Wheat heat-stressed seedling cDNA library
Tissue
Leaf
Developmental Stage
Two-week old seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0753_B05_D09ZS Wheat heat-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0753_B05_D09, mRNA sequence.
Other Name
WHE0753_B05_D09ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA015E1X
Clone
WHE0753_B05_D09
Probe
WHE0753_B05_D09
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Two-week old seedling were heat treated at 37 C for 2 hr and cyclic heat treated at 37 C for 2 hr for two days at Texas Tech University (D. Zhang, H. Nguyen lab). The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.28018.1.S1_at636WHE2AFFY
Ta.14200.1.S1_at62WHE2AFFY
Ta.14200.1.S1_at54WHE2AFFY
DNA
tcaaagaagttatccaatcaggcgttgttcctcgatttgtgcagtttctt
actagagaggatttcccccaactccagtttgaagcagcgtgggcactcac
aaacattgcgtctggcacatcanataataccaaggttgtcattgacgtng
ggcagttccaatatttgtgaagcttcttgcatctggcagcgatgatgttc
gtgagcacgctgtctgggcattgggcaatgttgctggtgactccccaaag
tgccgcgaccttgtactcgcaaatggtgcattgatgcctctgctagcaca
gttgaatgagcatgctaaactctccatgttaaggaatgccacctggactt
tatcaaatttct

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