GrainGenes Sequence Report: BE492869
Sequence
BE492869
Contig
TaAffx.121446.1.S1_at TaAffx.121446.1.S1_s_at TaAffx.121446.2.S1_s_at TaAffx.121446.3.S1_s_at NSFT03P2_Contig13161
Tracefile
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External Databases
Data at GenBank Data at EMBL Data at DDBJ
DB Remark
Locus Source: Triticum monococcum
Keyword
EST
Germplasm
T. monococcum ssp. monococcum DV92
Species
Triticum monococcum
Cultivar
DV92
Clone Library
Triticum monococcum vegetative apex cDNA library
Tissue
Vegetative shoot apex
Developmental Stage
Three weeks-old plants
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0565_H07_H07ZE Triticum monococcum vegetative apex cDNA library Triticum monococcum cDNA clone WHE0565_H07_H07, mRNA sequence.
Other Name
WHE0565_H07_H07ZE  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli XLOLR
DNA Library
TM011XXX
Clone
WHE0565_H07_H07
Probe
CFE245
Remark
DB_xref: taxon:4568 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene T3 primer. Note: Vector: Lambda pBK-CMV (Lambda Zap Express), excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova , J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda pBK-CMV (Lambda Zap Express), excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
gcttcaacaaatacaatgcttgtttgaatgcaatggctactcgtatatct
acagtatttgcatcaatgagggaatttccccgtgtgcactaccgcattgc
caagaccattgatgcttctacaatgacaacactaagggacatggtgccca
caaagattgctgctggtgtatggaattaccttt

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BE492869
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