GrainGenes Sequence Report: BE491998

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Sequence

BE491998

Tracefile

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External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Triticum monococcum

Keyword

EST

Germplasm

T. monococcum ssp. monococcum DV92

Species

Triticum monococcum

Cultivar

DV92

Clone Library

Triticum monococcum vegetative apex cDNA library

Tissue

Vegetative shoot apex

Developmental Stage

Three weeks-old plants

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE0555_E07_E07ZE Triticum monococcum vegetative apex cDNA library Triticum monococcum cDNA clone WHE0555_E07_E07, mRNA sequence.

Other Name

WHE0555_E07_E07ZE [ wEST-mySQL (obsolete) ]

Strain

lab_host E. coli XLOLR

DNA Library

TM011XXX

Clone

WHE0555_E07_E07

Remark

DB_xref: taxon:4568

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene T3 primer.

Note: Vector: Lambda pBK-CMV (Lambda Zap Express), excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova , J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

Note: Vector: Lambda pBK-CMV (Lambda Zap Express), excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

ggcacgagaagaaaacaatgaactgaagataaaactgaaggaagctgaaa
gttgtgccgagctcagcgagccgactacagaccccgagcacagcggaaga
gatttgagagcagagatacggaagctaaagaaagcctataaggccctgag
ctcgcaaaaggagagtgaggtttccgcgttacttgcagaaaaggattttg
tgtggaaccagtacaagacaatggagaaggattacgagacgctccttaag
aagaagaagatggaggcagcgcaggctaccgaagcagcagagaagcttca
gcggaaggtggaggagctgcaactgcaagtggtggcccaaaagaaggatg
acgatgttggcagattgcaagcggaagctaatgatgc


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