Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Sequence Report: Ta.1967.2.A1_x_at

[Submit comment/correction]

Sequence	BE490728
Contig	Ta.1967.2.A1_x_at TaAffx.104812.1.S1_s_at NSFT03P2_Contig15436
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC318873
wEST map position	BE490728
NCBI UniGene	Ta.64879
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001067015.1 Os12g0560200 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	5AS 5BS 5DS 7AS 7DS
Clone Library	Wheat cold-stressed seedling cDNA library
Tissue	Seedling
Developmental Stage	Five-day old seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0369_B09_D17ZS Wheat cold-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0369_B09_D17, mRNA sequence.
Other Name	WHE0369_B09_D17ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA007E1X
Clone	WHE0369_B09_D17
Probe	WHE0369_B09_D17
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were transferred to 5 C cold room and kept for 48 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were transferred to 5 C cold room and kept for 48 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	TaAffx.104812.1.S1_s_at 793 WHE2AFFY TaAffx.104812.1.S1_s_at 577 WHE2AFFY Ta.485.1.A1_at 80 WHE2AFFY Ta.485.1.A1_at 64 WHE2AFFY TaAffx.109337.1.S1_at 62 WHE2AFFY
DNA	caccccgtctaccgcctgctgcacccgcacttccgcttcaccatggagat caacgcgcaggcgcgcgcgatgctcatcaacgccgacgggatcatcgagg gctccttcgcgcccggggagtactccattgagctcagctccgtggcttac gaccagcagtggcggttcgacatggaggccctgccggaagacctcatccg gaggggcatggcggtcaggaaggagaatggcgagctggagctggccatag aggactacccctacgccaacgacggcctgctcatctgggacgccatcaag gagtgggcgttgacctacgtggagcactactacccgtgcaccgcggacat cgtcgatgacgaggagctccaggcttggtggaccgaggtgcgcaccaaag gccacgccgacaagcaggacgagccgtggtggcccgagctggacagccat gagaacctggcgcaggccctggcgaccatcatgtgggtcacgtcggcgca tcacg

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.