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GrainGenes Sequence Report: NSFT03P2_Contig2670

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Sequence	BE490497
Contig	NSFT03P2_Contig2670
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC290184
wEST map position	BE490497
NCBI UniGene	Ta.32361
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001049649.1 Os03g0265500 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	4DL
Clone Library	Wheat cold-stressed seedling cDNA library
Tissue	Seedling
Developmental Stage	Five-day old seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0362_H09_P18ZS Wheat cold-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0362_H09_P18, mRNA sequence.
Other Name	WHE0362_H09_P18ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA007E1X
Clone	WHE0362_H09_P18
Probe	WHE0362_H09_P18
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were transferred to 5 C cold room and kept for 48 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were transferred to 5 C cold room and kept for 48 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	TaAffx.129322.1.S1_at 72 WHE2AFFY TaAffx.129322.1.S1_x_at 72 WHE2AFFY Ta.6322.2.S1_a_at 64 WHE2AFFY Ta.6322.2.S1_x_at 64 WHE2AFFY
DNA	ccctgggaccgcgacgccatctcgctcgtctgccgccactggtgcaaggt cgacgcgctcagccgcaagcacgtcaccgtcgccatggcctactccacca cccccgaccgcctcttccgccgcttcccctgcctcgagtcgctcaagctc aaggccaagccccgcgcctccatgttcaacctcatccccgaggactgggg aggctccgcctcgccctggatccgcgagctctccncctccttccacttcc tcaaggggctgctcctccgccggatgattgtctcc

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