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GrainGenes Sequence Report: Ta.13275.2.S1_a_at

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Sequence
BE490196
Contig
Ta.13275.1.S1_at
Ta.13275.2.S1_a_at
NSFT03P2_Contig9932
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC292209
NCBI UniGene
Ta.13275
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001043613.1 Os01g0622700 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat cold-stressed seedling cDNA library
Tissue
Seedling
Developmental Stage
Five-day old seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0366_E03_J06ZS Wheat cold-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0366_E03_J06, mRNA sequence.
Other Name
WHE0366_E03_J06ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA007E1X
Clone
WHE0366_E03_J06
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were transferred to 5 C cold room and kept for 48 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were transferred to 5 C cold room and kept for 48 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tcgctaccttgcttcctcctgacgtgccttggcctccccgcttcccgttc
ccaccgacgacaggagcgagcagaggaccgcgatgcagcagccgcagctg
caggcgccgccctcgtgggcgtccgggcccgaccccgcccgccctgctcc
caccaccttcgacgaggcgtccatggagcggatcaagagctttgtcaagg
cgctccaggagctcaagaacctgcgcccacagctttactccgccgcggag
tactgcgagaagtcgtacctccacagcgagcagaagcatattgtgctgga
caacttgaaggattatgctgttagggctctggtcaacgcggtcgaccacc
ttggcaccgttgcctacaaattgacagacctgtatgaacaacaggtttca
gaggtctcaactgtcgagctgaaagtagcatccttgaaccagcaagtcct
gacttgc

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