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GrainGenes Sequence Report: BE489511

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Sequence	BE489511
Contig	NSFT03P2_Contig17830
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC282916
NCBI UniGene	Ta.54512
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001045534.1 Os01g0971500 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Clone Library	Wheat unstressed seedling shoot normalized cDNA library
Tissue	Etiolated shoot
Developmental Stage	Five day old seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE1059_B11_C21ZS Wheat unstressed seedling shoot normalized cDNA library Triticum aestivum cDNA clone WHE1059_B11_C21, mRNA sequence.
Other Name	WHE1059_B11_C21ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli DH10B
DNA Library	TA006E2N
Clone	WHE1059_B11_C21
Probe	[Wcl41ct186cn284] {SpCl-296} BE489511
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares'. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	gaggaggaggaggaggaggaggagaccatgtcgtcgtccggtggcaagct cttcaccctggaggaggtcgccaagcacagctccaaggacgactgctggc tcgtcatcggcggcaaggtgtacaatgtgaccaagtttcttgatgaccac cctggaggcgacgacgtcttgctgtcttcaactgccaaggacgcgaccga cgacttcgaggacgtgggacacagcacaaccgcccgggcgatgatggacg agtactacgtcggcgagatcgacgcgaccacgatacccaccaaggtcaag tacacgccgcccaagcagccgcactacaaccaggacaagacccccgagtt cgtcatcaagatcctccagttcttggtccccctcgcgatactgggcctgg ccgtcgccgtacggatctacaccaaggcagactctgtctagcaccgtcat cttataccagtcagtcagtcattcggaagtgtccaagagctgaataagtt ttagtggggatttgcatattttgtaccagcgtcaccgtcctgtcatggaa tctgggaactgctctcgacccgtcgttgcgttatg

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