GrainGenes Sequence Report: NSFT03P2

UniGene title 'Transcribed locus, moderately similar to NP_195437.1 HCF164 (High chlorophyll fluorescence 164); thiol-disulfide exchange intermediate [Arabidopsis thaliana]'

Keyword

EST

Germplasm

Chinese Spring

Species

Triticum aestivum

Cultivar

Chinese Spring

Clone Library

Wheat unstressed seedling shoot normalized cDNA library

Tissue

Etiolated shoot

Developmental Stage

Five day old seedling

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE1071-1074_F03_F03ZS Wheat unstressed seedling shoot normalized cDNA library Triticum aestivum cDNA clone WHE1071-1074_F03_F03, mRNA sequence.

Other Name

WHE1071-1074_F03_F03ZS [ wEST-mySQL (obsolete) ]

Strain

lab_host E. coli DH10B

DNA Library

TA006E2N

Clone

WHE1071-1074_F03_F03

Probe

[Wcl894ct1762cn2354]

{SpCl-456}

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares'. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

gagccgaacgtaactgaaaatggcgagcgtgacctcgaggtgctccggcc
tcctcctcccggacctgcagccgggcctcgcgggacttcgcagccgctcg
cgtccgccttcttcgccccgcgtcagtctgcgcgggggccgtcgccggcc
gagaaccctgtcctgcgtcgcgccgcccgactccgcggagccgaagacgg
acgagcagaacgtcaaagctgagctgggtgaagaaaaacctcagccaagc
atcagcagcagcagcagcacgcctcaggaggctgctgcagagccacctgt
tccggacaggggtctcaaccgaaggatagctgtgctcaccactcttgctg
cggtgggcctatttggatctcagaggctccagcttggcgggttttctctc
aaagatcttgctgccaatgctgtgccatacgaagaggctctctcaaatgg
caagcccactgttgtcgaattttacgcagactggtgtgaagtttgtcgag
agttagctc


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