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GrainGenes Sequence Report: BE489218

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Sequence	BE489218
Contig	Ta.1404.1.S1_at NSFT03P2_Contig11577
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC298392
wEST map position	BE489218
NCBI UniGene	Ta.1404
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Ribulose bisphosphate carboxylase activase B'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	4DS
Clone Library	Wheat unstressed seedling shoot normalized cDNA library
Tissue	Etiolated shoot
Developmental Stage	Five day old seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE1075_D07_G13ZS Wheat unstressed seedling shoot normalized cDNA library Triticum aestivum cDNA clone WHE1075_D07_G13, mRNA sequence.
Other Name	WHE1075_D07_G13ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli DH10B
DNA Library	TA006E2N
Clone	WHE1075_D07_G13
Probe	BE489218 MAG1418 WHE1075_D07_G13
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares'. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	Ta.1404.1.S1_at 771 WHE2AFFY [ Show all 6 ]
DNA	attcggcacgagggctggtggacaccttcccggggcagtccatcgacttc ttcggcgcgctgcgggcgcgggtgtacgacgacgaggtgcgcaagtgggt cggcgagatcggcgtggagaacatctccaagcggctcgtcaactccaggg aggggccgccgacgttcgaccagcccaagatgaccatcgagaagctcatg gagtacggccacatgctggtccaggagcaggagaacgtgaagcgcgtgca gctcgccgacaagtacctcagcgaggcggcgctcggccaagccaacgacg acgccatgaagaccggcgccttctacggcaagtagaatgaaggccagccc gtcctaattaagatgcatgcatgcatgcatgcactatatatatgttggaa tattttggactcaaactttttgattcatcgccgcacaggtaacccaattg taagtctcttgttgaaagagaatcgaattgtatatgttattgcctattta tacctgaatca

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