GrainGenes Sequence Report: NSFT03P2_Contig11434
Sequence
BE489053
Contig
Ta.20447.1.S1_at Ta.20447.1.S1_s_at NSFT03P2_Contig11434
Tracefile
[ Download ] [ View ]
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC289108
wEST map position
BE489053
NCBI UniGene
Ta.20447
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001047916.1 Os02g0714000 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
6AL 6BL 6DL
Clone Library
Wheat unstressed seedling shoot normalized cDNA library
Tissue
Etiolated shoot
Developmental Stage
Five day old seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE1061_B12_D23ZS Wheat unstressed seedling shoot normalized cDNA library Triticum aestivum cDNA clone WHE1061_B12_D23, mRNA sequence.
Other Name
WHE1061_B12_D23ZS [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli DH10B
DNA Library
TA006E2N
Clone
WHE1061_B12_D23
Probe
WHE1061_B12_D23
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares'. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.20447.1.S1_s_at 434 WHE2AFFY Ta.20447.1.S1_s_at 226 WHE2AFFY Ta.20447.1.S1_at 434 WHE2AFFY Ta.20447.1.S1_at 84 WHE2AFFY
DNA
ttggttcaaatcctgtcctggtgggggattgtgatcgtcgatgtttagat
acagcattattattgattctgtatctgacaagtcatggatatatggtgga
tgctggaatggagtccagcactctgtaaggttgactaatccaaggggatt
agtcatgatggcactcttaagaatcagggatgagtcacgatttgatggca
ctgagatgagcatccatctgaagagtcaccaagttagtgtgctttagtgt
cgcatagtttgagtcttgca
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig11434
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
| |||||||||||||
|
|
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||