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GrainGenes Sequence Report: BE488921

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Sequence	BE488921
Contig	Ta.27728.1.S1_at NSFT03P2_Contig16956
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC279376
wEST map position	BE488921
NCBI UniGene	Ta.54718
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Triose phosphate translocator'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	3BS 3DS
Clone Library	Wheat unstressed seedling shoot normalized cDNA library
Tissue	Etiolated shoot
Developmental Stage	Five day old seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE1077_F06_L11ZS Wheat unstressed seedling shoot normalized cDNA library Triticum aestivum cDNA clone WHE1077_F06_L11, mRNA sequence.
Other Name	WHE1077_F06_L11ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli DH10B
DNA Library	TA006E2N
Clone	WHE1077_F06_L11
Probe	CFE195 CFE196 WHE1077_F06_L11
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares'. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	Ta.27728.1.S1_at 1063 WHE2AFFY Ta.27728.1.S1_at 139 WHE2AFFY TaAffx.122974.1.S1_at 216 WHE2AFFY TaAffx.122974.1.S1_at 194 WHE2AFFY Ta.4449.1.A1_at 48 WHE2AFFY
DNA	caattcggcacgaggttggtctcccgaacgtgcgcctatcaatgcaacgc tcctgaagctgctctttccggtggcattgtgccatgctcttggtcatgtc acaagcaacgtgtcctttgctactgttgcagtctcgtttgcccacactat taaagctttggagcccttcttcaatgcagctgctacccagtttgtccttg gacagacagttcccttgtctctgtggttgtctcttgcccctgttgtgctt ggtgtttcaatggcatccctcactgaactgtcgtttagctggaagggttt catcaatgctatgatctctaacatctcgttcacttaccggagtatttatt ccaagaaagccatgactgatatggatagcaccaacgtgtatgcttacatc tcaataattgctctagttgtctgcataccacctgcacttattattgaagg accacaactaatgcagtacggattaaacgatgcaattgcaaaagtaggaa tgacaaaatttgtatcagatcttttcctggtgggattgttctaccatctc tataatcagattgctacaaacactttggagcggg

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