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GrainGenes Sequence Report: NSFT03P2_Contig16079

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Sequence
BE488825
Contig
Ta.20429.2.S1_at
NSFT03P2_Contig16079
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC350143
wEST map position
BE488825
NCBI UniGene
Ta.32936
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_001065862.1 Os11g0171300 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
5AS
5BS
Clone Library
Wheat unstressed seedling shoot normalized cDNA library
Tissue
Etiolated shoot
Developmental Stage
Five day old seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1078_G03_N06ZS Wheat unstressed seedling shoot normalized cDNA library Triticum aestivum cDNA clone WHE1078_G03_N06, mRNA sequence.
Other Name
WHE1078_G03_N06ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli DH10B
DNA Library
TA006E2N
Clone
WHE1078_G03_N06
Probe
MAG1726
WHE1078_G03_N06
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares'. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.20429.2.S1_at712WHE2AFFY
[ Show all 11 ]
DNA
cgccgactacaccctcaagctcctcagccgccgcgtgccgcccgccgtcc
ccggcatcatgttcctctcgggagggcagtccgaggtggaggccacgctc
aacctcaatgccatgaaccaggggcccaacccgtggcacgtctccttctc
ctacgcccgggcgctgcagaacacgtgcctcaagacgtggggcggccggc
cggagaacgtcaaggcggcgcaggaggcgctgctgctgcgtgccaaggcc
aactcgctcgcgcagctcggaaagtacaccagcgacggcgaagccgccga
ggccaaggagggcatgttcgtcaagaactacagctactaatccaaccatt
ccaatcaacactactacgtacgtacgttcgttaccaaagcagaaattaaa
gatgcatgcatgatgattcagctgcaagtgcaagatcgatcgagctggac
cctcagtctgagatgatgattacttgtattttctagatctttttttactc
gttaatttgcctctcgatatgcacaattcatgctacagtccagtagatta
ttgtaaagctacttttacgtgtgcaatccatggatttgcatggttactgc
aaaaaaaaaaaaaaaaaaaa

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