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GrainGenes Sequence Report: BE471268

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Sequence
BE471268
Contig
NSFT03P2_Contig15067
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC335544
wEST map position
BE471268
NCBI UniGene
Ta.7803
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, strongly similar to NP_001050053.1 Os03g0338000 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
4AS
4DL
Clone Library
Wheat drought-stressed seedling cDNA library
Tissue
Seedling without endosperm
Developmental Stage
Five day old seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0286_B11_D22ZS Wheat drought-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0286_B11_D22, mRNA sequence.
Other Name
WHE0286_B11_D22ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA005E1X
Clone
WHE0286_B11_D22
Probe
WHE0286_B11_D22
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were incubated for one day at 90% RH. After removing endosperm, seedlings were transferred to desiccator jar containing saturated MgSO4 at room temperature for 24 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were incubated for one day at 90% RH. After removing endosperm, seedlings were transferred to desiccator jar containing saturated MgSO4 at room temperature for 24 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.6723.1.S1_x_at56WHE2AFFY
Ta.6723.3.S1_a_at56WHE2AFFY
Ta.4834.1.S1_at54WHE2AFFY
Ta.4834.1.S1_at50WHE2AFFY
DNA
tcaagggaagtgaatcacatcgggtgatcgcaacatattggattattcgt
tccattcaggtgggcagtagtgatgctgggggctaggagatccttctcta
ggctcgctgcggcggttaggacgccggtggacgtgccgagaatgccggcg
ttcgaccacgtgccgctgccctacgacgggccgagcgccgtcgagatcgc
caggaagcgcgccgagttcctcagcccctccctgttccatttctactcca
agcccctcaacattgtggaggggaagaaacagtacctctacgacgagcac
gggcggcgctacctggacgcgttcgccggcatcgccaccgtgtgctgcgg
ccacacccaccccgacatcatcgacgccatcaccgcgcaggccaaccgcc
tgcagcactccaccgtgctctacctcaaccacgccatcgcagactttgcc
gaggcgctggccgccaagatgcccggcgatctcaaggtcgtcttcttcac
caactccggcacggaggccaacgagctcgccatcatgatggcgag

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