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GrainGenes Sequence Report: NSFT03P2_Contig10463

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Sequence	BE471238
Contig	Ta.28322.1.S1_s_at NSFT03P2_Contig10463
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC289498
NCBI UniGene	Ta.49756
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Clone wre1n.pk0035.f6:fis, full insert mRNA sequence'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Clone Library	Wheat drought-stressed seedling cDNA library
Tissue	Seedling without endosperm
Developmental Stage	Five day old seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0285_B01_D01ZS Wheat drought-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0285_B01_D01, mRNA sequence.
Other Name	WHE0285_B01_D01ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA005E1X
Clone	WHE0285_B01_D01
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were incubated for one day at 90% RH. After removing endosperm, seedlings were transferred to desiccator jar containing saturated MgSO4 at room temperature for 24 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were incubated for one day at 90% RH. After removing endosperm, seedlings were transferred to desiccator jar containing saturated MgSO4 at room temperature for 24 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	gatccaagactaggccggccggccggaggagatcgagaaggaggaggaga catggtgcgcgggaagctggaggtgctgctcgtctccgccaagggcctcg acgactccgatttcttcaatagcatggacccgtacgtgatcctcacctgc cgcagccacgagcagaagagcaccgtcgcatcaggagcagggagcgagcc tgagtggaacgagaccttcgtcttcgccgtctccggcgacgctccggagc tcagggtaaagatcatggacagcgacgccctctcggccgacgacctcgtc ggagaagcatgtatcccgctggaggctgtgctccaggagggcagcctgcc gccggccgtgcaccgggtcgtcaaggacgaggagtaccgcggggagatca agatcgcgctcaccttcaccccggcagaggaaaacgaggaggaggangga gagctacggcggctggaatcagtccacctgaaaaaggccagcgagccagc aagatggtgctgtatgtctgactgtcataatggatagaaaggctttggat atccttgatgtgtgtgac

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