GrainGenes Sequence Report: Ta.4760.1.S1_x

UniGene title 'Transcribed locus, moderately similar to NP_851200.1 ACA8 (AUTOINHIBITED CA2+ -ATPASE, ISOFORM 8); calcium-transporting ATPase/ calmodulin binding [Arabidopsis thaliana]'

Keyword

EST

Germplasm

Chinese Spring

Species

Triticum aestivum

Cultivar

Chinese Spring

Clone Library

Wheat drought-stressed seedling cDNA library

Tissue

Seedling without endosperm

Developmental Stage

Five day old seedling

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE0280_D02_G04ZS Wheat drought-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0280_D02_G04, mRNA sequence.

Other Name

WHE0280_D02_G04ZS [ wEST-mySQL (obsolete) ]

Strain

lab_host E. coli SOLR

DNA Library

TA005E1X

Clone

WHE0280_D02_G04

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were incubated for one day at 90% RH. After removing endosperm, seedlings were transferred to desiccator jar containing saturated MgSO4 at room temperature for 24 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were incubated for one day at 90% RH. After removing endosperm, seedlings were transferred to desiccator jar containing saturated MgSO4 at room temperature for 24 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

gggctgcagcattcggctcgagttactggaaagagctgctgaacttattt
tgcagtcatgcactagttggattggcacggatggttcaaagcaatcaatg
acccctgagaaagttggtgagttcaagaaattcattgaagacatggctgt
tgctagccttcgctgcgtcgcctttgcttacaggccttgtgagatgagtg
atgttccaaaggaggaccagagggctgactgggtattacctgaggataac
ctgattatgcttgggattgtgggaataaaggatccctgccgccctggagt
tcaagattctattcgcttgtgtgccgcagctggcattaaggtccgcatgg
tcactggggacaatcttcaaactgccagagcgattgccttggaatgtggt
attcttactgatcctaacgtatcagagccaaccatcattgaagggaagac
cttccgggagttgtcagatttggaaagggaggaagtcgccgacaaaatct
ctgttatggggaggtcttcaccgaacgataaacttctacttgttaaggca
ctcagaaacagaggtcatgttgttgctgtaactggtgatggcacaaacga
tgccccagcactgcatgaggccgacattggtctctcaatgggcatc


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