Query (optional)   in Class  

GrainGenes Sequence Report: NSFT03P2_Contig9913

[Submit comment/correction]

Sequence
BE446546
Contig
Ta.13535.1.S1_at
NSFT03P2_Contig9913
Tracefile
[ Download ]
[ View ]
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC342255
wEST map position
BE446546
NCBI UniGene
Ta.13535
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
2AS
2BL
6AL
Clone Library
Wheat etiolated seedling root normalized cDNA library
Tissue
Root
Developmental Stage
Five day old etiolated seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1456_B01_C02ZS Wheat etiolated seedling root normalized cDNA library Triticum aestivum cDNA clone WHE1456_B01_C02, mRNA sequence.
Other Name
WHE1456_B01_C02ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli DH10B
DNA Library
TA008E3N
Clone
WHE1456_B01_C02
Probe
MAG1458
WHE1456_B01_C02
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.13535.1.S1_at702WHE2AFFY
Ta.13535.1.S1_at511WHE2AFFY
DNA
gggcagcggtggtggtgttggcggttctcgggctagcgtacgcggtggcg
cggcgccgcgtgggcgtcgcgcaaggtgacgctgaggcggctagcgcacc
gaagtcgaatgcttaatgggctggattcggtacgcacgcatgcgtgtgga
tggatggactttgcgtgggcctagagagttaagccataagggcgggctgg
tagtgtgtaaattatcagtgtttcatcgtcgagcgacgggaaaatggttg
caggcgacatccactgtctgagctctcaagcctacggccgatcgtgctgt
gaacaatcatcacgtactaataacctcagagaacgcttttgttcgagtga
aatgcacctataggcgttaaattaaaaaaaaaaaaaaaaaaa

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.