GrainGenes Sequence Report: BE446207

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Sequence

BE446207

Contig

NSFT03P2_Contig11500

Tracefile

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External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Transcribed locus, weakly similar to NP_001047893.1 Os02g0709900 [Oryza sativa (japonica cultivar-group)]'

Keyword

EST

Germplasm

Chinese Spring

Species

Triticum aestivum

Cultivar

Chinese Spring

Clone Library

Wheat etiolated seedling root normalized cDNA library

Tissue

Root

Developmental Stage

Five day old etiolated seedling

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE1142_A11_A22ZS Wheat etiolated seedling root normalized cDNA library Triticum aestivum cDNA clone WHE1142_A11_A22, mRNA sequence.

Other Name

WHE1142_A11_A22ZS [ wEST-mySQL (obsolete) ]

Strain

lab_host E. coli DH10B

DNA Library

TA008E3N

Clone

WHE1142_A11_A22

Probe

[Wcl947ct1821cn2417]

{SpCl-1397}

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

cccatgacaccatcaggaagtgagcgtgggggcgtcgaagatgatgatga
tgatgattcagagaggcgaaacaaatcgattgggaaaacgctatggtctt
tcttcacgacataacaaga


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