GrainGenes Sequence Report: BE446029
Sequence
BE446029
Contig
Ta.28753.2.S1_a_at NSFT03P2_Contig1785
Tracefile
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External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC361860
wEST map position
BE446029
NCBI UniGene
Ta.55918
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_564870.1 ATX1; metal ion binding [Arabidopsis thaliana]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
7AL 7BL 7DL
Clone Library
Wheat etiolated seedling root normalized cDNA library
Tissue
Root
Developmental Stage
Five day old etiolated seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE1148_B02_D04ZS Wheat etiolated seedling root normalized cDNA library Triticum aestivum cDNA clone WHE1148_B02_D04, mRNA sequence.
Other Name
WHE1148_B02_D04ZS [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli DH10B
DNA Library
TA008E3N
Clone
WHE1148_B02_D04
Probe
WHE1148_B02_D04
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.28753.2.S1_a_at 684 WHE2AFFY Ta.28753.2.S1_a_at 557 WHE2AFFY Ta.28753.1.S1_x_at 662 WHE2AFFY Ta.28753.1.S1_x_at 391 WHE2AFFY
DNA
ctcacccccacgtttcctttcccccctccctcgcgccctcgccgtcacgc
ggatcaaccgatctcgcacgcaatgtctcagactgttgaactcagggttg
gcatgtcatgcgagggatgcgttggagctgttaagcgggttcttggcaaa
atggaaggtgttgagtccttcgatgtagacatcaaggagcagaaggtcac
tgtgaagggtaatgtgacgccggatgctgttctgcagactgtttcgaaga
caggcaagaagacgtcgttctgggaggctgaaccctcggcatctgctgtt
tcgtcctaaaaaggcacctctaatatgtaaactgtgaagtttaatgtacc
aatggcccagttgaaatgctttgcaaggacatgattttctggtcggatgg
tatatgcaccatgaatttactgcaaataatagcaaactggtaatcgtttg
tgtgcatcttatggttggcaact
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BE446029
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