GrainGenes Sequence Report: BE445579
Sequence
BE445579
Contig
Ta.2138.1.S1_a_at NSFT03P2_Contig15259
Tracefile
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External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC298666
wEST map position
BE445579
NCBI UniGene
Ta.8471
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001055700.1 Os05g0449500 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1AL 1BL 1DL 3DL
Clone Library
Wheat etiolated seedling root normalized cDNA library
Tissue
Root
Developmental Stage
Five day old etiolated seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE1451_E04_I07ZS Wheat etiolated seedling root normalized cDNA library Triticum aestivum cDNA clone WHE1451_E04_I07, mRNA sequence.
Other Name
WHE1451_E04_I07ZS [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli DH10B
DNA Library
TA008E3N
Clone
WHE1451_E04_I07
Probe
WHE1451_E04_I07
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.2138.1.S1_a_at 797 WHE2AFFY Ta.6322.2.S1_a_at 212 WHE2AFFY Ta.6322.2.S1_x_at 212 WHE2AFFY Ta.6322.1.S1_x_at 176 WHE2AFFY
DNA
accctggcatgcaagaagaggaaggcggagtttctcaagtaggcctaaca
gccatagccgtaggttgccgtgagctggaaaacatagctgcctatgtgtc
tgatatcacaaatggggccctggaatccatcggaacgttctgcaaaaatc
tccatgactttcgccttgtcctgcttgacaaacaagagacgataacagat
ttgccgctggacaacggtgcccgcgcgctgctcaggggctgcaccaagct
tcggaggttcgctctatacctgagaccaggggggctttcagatgtaggcc
tcggctacatcgggcagcacagtggaaccatccagtacatgcttctgggt
aacgtcgggcagacggatggtggattgatcagtttcgcagccgggtgccg
gaacctgcggaagcttgaactgaggagctgttgcttcatcgagcgggctt
tgg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BE445579
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