Query (optional)   in Class  

GrainGenes Sequence Report: BE445496

[Submit comment/correction]

Sequence
BE445496
Contig
Ta.23176.1.S1_at
NSFT03P2_Contig13950
Tracefile
[ Download ]
[ View ]
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC290577
wEST map position
BE445496
NCBI UniGene
Ta.23176
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Protein phosphatase 2C'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
3AL
3BL
3DL
Clone Library
Wheat etiolated seedling root normalized cDNA library
Tissue
Root
Developmental Stage
Five day old etiolated seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1135_D03_H05ZS Wheat etiolated seedling root normalized cDNA library Triticum aestivum cDNA clone WHE1135_D03_H05, mRNA sequence.
Other Name
WHE1135_D03_H05ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli DH10B
DNA Library
TA008E3N
Clone
WHE1135_D03_H05
Probe
WHE1135_D03_H05
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.23176.1.S1_at1112WHE2AFFY
TaAffx.58269.1.S1_at333WHE2AFFY
TaAffx.58269.1.S1_at272WHE2AFFY
Ta.26745.1.A1_at186WHE2AFFY
Ta.26745.1.A1_at98WHE2AFFY
DNA
ggcacaagcagtgtcactgcaatgggcatggtagatcctgtagcacccga
gaccgtcggttcaacggcggtggtcgctgtcatctgctcctctcatatca
ttgtctcaaattgtggagactcgagggcagtgctctgccgtggcaagcaa
cccgtgccgttgtcagtggatcataaacctaatagggaggatgagtacgc
aaggattgaggcagagggtggcaaggtcatacagtggaatggctaccgag
ttttcggtgtccttgccatgtcgcggtcaattggtgacagatatctgaaa
ccatggataattcctgtcccagaggtcacaattgttcctcgagcaaagga
tgacgagtgtcttattctcgctagtgatggcctctgggatgtactgtcga
atgaagaggtatgcgatgttgcccgcaagcgaatactcttatggcataaa
aagaatggggtaaacttgtcatcggcccaacgtagcggtgactccccaga
tccagcggctcaagcagctgctgaatgcttgtcgaagcttgctctccaga
aggggagcaaggacaacatcacggttattgtggta

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.