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GrainGenes Sequence Report: NSFT03P2_Contig13189

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Sequence	BE443977
Contig	Ta.1576.1.S1_at NSFT03P2_Contig13189
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC312148
NCBI UniGene	Ta.1576
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Clone Library	Wheat etiolated seedling root normalized cDNA library
Tissue	Root
Developmental Stage	Five day old etiolated seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE1123_F11_L20ZS Wheat etiolated seedling root normalized cDNA library Triticum aestivum cDNA clone WHE1123_F11_L20, mRNA sequence.
Other Name	WHE1123_F11_L20ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli DH10B
DNA Library	TA008E3N
Clone	WHE1123_F11_L20
Probe	[Wcl843ct1707cn2291] {SpCl-298} BE443977
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	ccagcgcttactggtcaggtgaagtggttcaacgagagcaaggggttcgg ctttatcacccccgacgacggcagcaaggacgtcttcgtgcacttctcgg caatcgttgacgccggcttcaagaccctcgccgagggccagcgtgtcgaa ttcagaatcgaaagcggcgcaaaaggcccgtccgccgccaatgtcaaggc catataggaggaggagcaatatcggcatccttttgtaatttgctccaccc gaccgagcaaatggagtccaagagtatgctgctttaggaaggattgttat ttgtttgtgttctattctcaccagtgcactaaaatctgtactgtttcttt cttttcttttcttttcttttcacatcaaatctacacgggttaattactgt tgcgttcgggtttgcaaggaatgtggattttgtttaagtcaaatctttaa acttcgaataagtttgtacaaagatgtactgtatcaatattcacagtacc aaatcaatatcaataaattatcatgaaatctcttcttataaaaaaaaaaa aaaaaaaaaact

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