GrainGenes Sequence Report: BE426885
Sequence
BE426885
Contig
TaAffx.112507.1.S1_at NSFT03P2_Contig3308
External Databases
Data at GenBank Data at EMBL Data at DDBJ
NCBI UniGene
Ta.37279
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001055367.1 Os05g0373400 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat unstressed seedling shoot cDNA library
Tissue
Etiolated shoot
Developmental Stage
Five day old seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0333_D04_G07ZS Wheat unstressed seedling shoot cDNA library Triticum aestivum cDNA clone WHE0333_D04_G07, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0333_D04_G07
Probe
[BE426885.1] {SpCl-1293}
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Shoots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Shoots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
catggcggaggacgccggcgacagggaccagcagcagcagcagcagcccg
ccgcggccgcggggcccggagcagggcatgcctgcggggaggacggcggc
ggcagggacgagagctccgtcaagctcttcgtcggccaggtgcccaagct
catgaccgaggccgagctggccgccatgttccgcgacgtcgccgtcgtcg
acgaggtcaccgtcatccgcgacaaggccaccaaggcgtcccgaggttgc
tgcttcctgatctgcccatcaagggaggaggctgacaaggcagtgaatgc
atatcacaacaaacgcacgcttcctggggccccaagtccattgcaagtaa
aatatgccgatggagagttggaaaggctggagcacaaacttttcatcgga
atgctccccaaaaatgtaacagatgctgaaatgactgatttattttcaca
atatggaaatatcaaagatttgcagattttgagaggttcacaacagacga
gcaaagccggctgtgcctttctgaag
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BE426885
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
| ||||||
|
|
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||