GrainGenes Sequence Report: TaAffx.85169.1.S1_at
Sequence
BE426725
Contig
TaAffx.85169.1.S1_at NSFT03P2_Contig10545
Tracefile
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External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC307872
NCBI UniGene
Ta.32294
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_195437.1 HCF164 (High chlorophyll fluorescence 164); thiol-disulfide exchange intermediate [Arabidopsis thaliana]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat unstressed seedling shoot cDNA library
Tissue
Etiolated shoot
Developmental Stage
Five day old seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0331_B07_D13ZS Wheat unstressed seedling shoot cDNA library Triticum aestivum cDNA clone WHE0331_B07_D13, mRNA sequence.
Other Name
WHE0331_B07_D13ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA006E1X
Clone
WHE0331_B07_D13
Probe
[Wcl894ct1762cn2354] {SpCl-456}
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Shoots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Shoots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
taactgaaaatggcgagtgtgacctcgaggtgctccggcctcctcctccc
ggacctgcggccgggcctcgcgggactccgcagccgctcgcgtccgcctt
cttcgccccgcgtcagtctgcgcgcgggccgtcgccggccgagaaccctg
tcctgcgtcgcgccgcccgactccgcggagcccaagacggacgagcagca
ggtgaaagctgagctgggtgaagaaaagcctcagccaagcagcagcagca
gcagcacgccccaggaggctgctgcagagccacctgttccagacaggggt
ctcaacagaaggatagctgtgctcaccactcttgccgcggtgggcctctt
tggatctcagaggctccaacttggcgggttttctctcaaggatcttgctg
ccaatgctgtgccatatgaagaggctctctcaaatggcaagcccactgtt
gtcgaattttacgcggactggtgtgaagtttgtcgagagttagctccaga
tatttacaaaattgagcaacagtacaaggaccgcatcaactttgtcatgt
tgaatgtcgacaatacaaagtgggaacaagagcttgatgagttt

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: TaAffx.85169.1.S1_at
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