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GrainGenes Sequence Report: BE425978

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Sequence	BE425978
Contig	NSFT03P2_Contig12017
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC309882
wEST map position	BE425978
NCBI UniGene	Ta.1358
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Clone wlm1.pk0015.c4:fis, full insert mRNA sequence'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	1DS
Clone Library	Wheat unstressed seedling shoot cDNA library
Tissue	Etiolated shoot
Developmental Stage	Five day old seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0326_C03_E06ZS Wheat unstressed seedling shoot cDNA library Triticum aestivum cDNA clone WHE0326_C03_E06, mRNA sequence.
Other Name	WHE0326_C03_E06ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA006E1X
Clone	WHE0326_C03_E06
Probe	WHE0326_C03_E06
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Shoots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Shoots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	Ta.1358.1.S1_at 751 WHE2AFFY Ta.1358.1.S1_at 244 WHE2AFFY
DNA	tggcttggaaaatattaatgttgttacacagcgtggttttattcctgttg atgagcggatgcaagtcacggatgctgatggcaatgtggtgccgaattta ttttgcattggagatgcgaatggtaaactcatgcttgcccatgctgccag tgcacagggaatctcagtcgttgagcaaatctctgggagggaccacattc taaaccatttaagcatcccagccgcttgtttcactcatccagaaatcagt atggttgggctgacagagccacaagccagagaaaaggctgacaacgaagg atttgaggtaagtgttgttaagaccagttttaaggccaacacaaaagctt tggcagaaaatgaaggcgatggaattgctaagatgatctacagacctgac acgggagaaattcttggtgatttggttcttgttgccttcatttctttgtt gtttcacctattgagttgtaagtctagccagggttcttcaaataatcggc gttgtcgccgcgatgtattttaagactgctcagttcagcaggatgatgtc tgaaaagaagtttgcaggttgaaggactccaaaaaaaaaaaaaaaaaaaa aactc

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