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GrainGenes Sequence Report: BE425942

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Sequence	BE425942
Contig	TaAffx.114847.1.S1_at NSFT03P2_Contig3397
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC305732
wEST map position	BE425942
NCBI UniGene	Ta.35331
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001059758.1 Os07g0510900 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	2BS 2DS
Clone Library	Wheat unstressed seedling shoot cDNA library
Tissue	Etiolated shoot
Developmental Stage	Five day old seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0326_F06_K12ZS Wheat unstressed seedling shoot cDNA library Triticum aestivum cDNA clone WHE0326_F06_K12, mRNA sequence.
Other Name	WHE0326_F06_K12ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA006E1X
Clone	WHE0326_F06_K12
Probe	WHE0326_F06_K12
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Shoots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Shoots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	TaAffx.114847.1.S1_at 446 WHE2AFFY TaAffx.114847.1.S1_at 335 WHE2AFFY TaAffx.114847.1.S1_at 278 WHE2AFFY Ta.1890.1.S1_x_at 86 WHE2AFFY TaAffx.90344.1.S1_at 82 WHE2AFFY
DNA	aatgccctcatgctgacgagtgacgactccaccaacataagaagaagagc acccatcccagtcacaagtccctcgcatctcctttccaatcctagttcca tacttccattgctgcaagaaagcgtctaggtaggcggcgcggtcagcaag atggcgaggggtggcagcgccgtggctgtcctctccttcctcctcggcct cccgctcctctccgtcctcgtcgccggcgaggatccgtaccggttcttca cgtggaacgtcagctacggcgacatctacccgctcggcgtcaagcagcag gggatactgatcaatgggcagttcccggggccacaggtagaggccgtcac caacgacaacctcgtcgtcaatgtcttcaacaagctcaacgagccattcc ttctctcctggagcggggtacagcagcggcggaactcgtttgaggacggc gtggccgggacgacgtgcccgatcccgccgggcgccaacttcacctacat cctgc

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