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GrainGenes Sequence Report: BE425904

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Sequence	BE425904
Contig	NSFT03P2_Contig3406
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC310222
wEST map position	BE425904
NCBI UniGene	Ta.622
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'AML15'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	4AL
Clone Library	Wheat unstressed seedling shoot cDNA library
Tissue	Etiolated shoot
Developmental Stage	Five day old seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0325_H02_O03ZS Wheat unstressed seedling shoot cDNA library Triticum aestivum cDNA clone WHE0325_H02_O03, mRNA sequence.
Other Name	WHE0325_H02_O03ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA006E1X
Clone	WHE0325_H02_O03
Probe	WHE0325_H02_O03
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Shoots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Shoots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	Ta.622.1.S1_at 103 WHE2AFFY
DNA	ctctgaactgcgctctctctttgagcaatttggggatatccggacccttt acactgcaaccaagcataggggctttgtgatgatatcttattttgatatc cgagctgcacgtggtgcgatgcgttcgttgcagaataagcctctgaggag gagaaagcttgacattcatttttcgatcccaaaggagaatccatctgaca aagatttaaaccaggggactcttgttatctttaacttggatccctcagtt tctaatgaagaagttcgccagatttttggaacttatggggaagtaaagga gataagagagacgcctaacaagaagcaccacaagtttattgagttctacg atgttagggcggcagaagctgctcttcggtcactgaataagagcgagatc gctggaaaacgcatcaagttggagccaagtcgtcctggtggaacacgcag aagcttggtgcaacaccttggtcatgaactagagcaagatgaacccagag gttacaggcattctcatgttggatcgcccatggccaactctcctccaggg gcatgggctcaatatggcagtccaaccgacaacaatttgctgcatgcatt cagcaactcacctactggaaatggaatgagt

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