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GrainGenes Sequence Report: Ta.27778.1.S2_at

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Sequence
BE423573
Contig
Ta.27778.1.S2_at
NSFT03P2_Contig18702
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC319490
NCBI UniGene
Ta.24085
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Alpha/beta-gliadin precursor'
Keyword
EST
Germplasm
CHEYENNE
Species
Triticum aestivum
Cultivar
Cheyenne
Clone Library
Wheat endosperm cDNA library
Tissue
Endosperm
Developmental Stage
5 to 30 days post anthesis seed
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0071_C02_E03ZS Wheat endosperm cDNA library Triticum aestivum cDNA clone WHE0071_C02_E03, mRNA sequence.
Other Name
WHE0071_C02_E03ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA001E1X
Clone
WHE0071_C02_E03
Probe
BE423573
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda ZAP II, excised phagemid; Site_1: EcoRI; Seeds collected, endosperm isolated, and RNA prepared by Susan Altenbach. Library constructed by Stratagene, Inc. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab.
DNA
ttttattcccaagtttgaactagtatagttcgggttcttacatgatagtg
cattgtcacattattttttcaccgctacaacgaccaaaccatggactgag
aaaacggtgtttcatatatctagtactagagttattttcttctcagttag
tatcgaagatgccaaatggcgtgatggtgcaatatggagggatgtagaca
ttgcacattgcaggtagcgtctgtagcgctaggttccttatttcctcgaa
ctggggcagttgttgaggctggacagagccctgggcctgtgggttttgct
gagatggccggaaggagccctggcctaatggatattgttgcagaggctgt
tggaaggagacctggctcgatggttgttgttgttgtttttgttgttgatg
cagaataatagcatgaacaactttgtggatggcctggcactgcgactgct
cagggatctgccataggtgctgacaacacaattcttgcaacagctggtaa
gtactttgttgcaaaacttgtgatcttccatgcgctatgttgtgttgctg
caatacaacatccatgcatggaatcagttgttgttgcaaaatttgtt

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