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GrainGenes Sequence Report: BE423265

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Sequence
BE423265
Contig
NSFT03P2_Contig18405
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC302155
NCBI UniGene
Ta.54206
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Pre-alpha-/beta-gliadin A-II'
Keyword
EST
Germplasm
CHEYENNE
Species
Triticum aestivum
Cultivar
Cheyenne
Clone Library
Wheat endosperm cDNA library
Tissue
Endosperm
Developmental Stage
5 to 30 days post anthesis seed
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0063_H05_O09ZS Wheat endosperm cDNA library Triticum aestivum cDNA clone WHE0063_H05_O09, mRNA sequence.
Other Name
WHE0063_H05_O09ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA001E1X
Clone
WHE0063_H05_O09
Probe
BE423265
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda ZAP II, excised phagemid; Site_1: EcoRI; Seeds collected, endosperm isolated, and RNA prepared by Susan Altenbach. Library constructed by Stratagene, Inc. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab.
DNA
acttaccagctggtgcaacaattgtgttgtcagcagctgtggcagatccc
cgagcagtcgcggtgccaagccatccacaatgttgttcatgctattattc
tgcatcaacaacaacaacaacaacaacaacaacaacaacaaccgttgagc
caggtctccttccaacagcctcaacaacaatatccatcaggccagggctc
cttccagccatctcagcaaaacccacaggcccagggctctgtccagcctc
aacaactgccccagtttgaggaaataaggaacctagcgctagagacgcta
cctgcaatgtgcaatgtctatatccctccatattgcaccattgctccagt
tggcttcttcggtactaactgagaagagaagaactctagtagtagacata
tgatacaccgttttcttagtccatggtttggtcgttgtagcggtgaaaaa
ataaagtgacatgcagtatcatgtaagaacccgaactatactagttcaaa
c

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