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GrainGenes Sequence Report: BE423089

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Sequence
BE423089
Contig
NSFT03P2_Contig18655
Tracefile
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External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC280985
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
EST
Germplasm
CHEYENNE
Species
Triticum aestivum
Cultivar
Cheyenne
Clone Library
Wheat endosperm cDNA library
Tissue
Endosperm
Developmental Stage
5 to 30 days post anthesis seed
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0060_B12_C24ZS Wheat endosperm cDNA library Triticum aestivum cDNA clone WHE0060_B12_C24, mRNA sequence.
Other Name
WHE0060_B12_C24ZS  [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA001E1X
Clone
WHE0060_B12_C24
Probe
BE423089
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda ZAP II, excised phagemid; Site_1: EcoRI; Seeds collected, endosperm isolated, and RNA prepared by Susan Altenbach. Library constructed by Stratagene, Inc. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab.
DNA
gtgctcaatacaaatccaccatgaagacctttctcatccttgccctcctt
gctattgtagcaaccaccgccacaattgcagttagagttccagtgccaca
attgcagccacaaaatccatctcagcaacaaccacaagagcaagttccat
tggtacaacaacaacaatttccagggcagcaacaaccatttccaccacaa
cagccatatccgcagccgcaaccatttccatcacaacaaccatatctgca
gctgcaaccatttccgcagccgcaactaccatatccgcagccgcaactac
catatccgcagccgcaactaccatatccgcagccgcaaccatttcgacca
caacaaccatatccacaatcgcaaccacagtattcgcaaccacaacaacc
aatttcgcagcagcagcagcaacaacaacaacaacaacaaaaacaacaac
aacaacaacaacaacaacaacagatccttcaacaaattttgcaacaacaa
ctgattccatgcagggatgttgtattgcaacacacagcatagcgtatgga
agctcacaagttttgcaacaaagtactta

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