GrainGenes Sequence Report: NSFT03P2_Contig15799
Sequence
BE406474
Contig
NSFT03P2_Contig15799
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC324633
wEST map position
BE406474
NCBI UniGene
Ta.54610
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001050088.1 Os03g0345200 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
2AS 2BS 2DS
Clone Library
Wheat etiolated seedling root cDNA library
Tissue
Root
Developmental Stage
Five day old etiolated seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0416_g09_n18zB Wheat etiolated seedling root cDNA library Triticum aestivum cDNA clone WHE0416_g09_n18, mRNA sequence.
Other Name
WHE0416_g09_n18zB [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA008E1X
Clone
WHE0416_g09_n18
Probe
WHE0416_g09_n18
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Strategene pBluescript SK reverse primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.2887.1.S1_x_at 414 WHE2AFFY
[ Show all 13 ]
DNA
aggccgcacccgtccgtctcctccctccgccgagcagcgtcccagtatcc
ctccagtccccattatgcagaacgaggtgggtgatatggtggacctctat
gtcccaaggaagtgctctgccacgaacaggatcataactgccaaggacca
cgcctctgtccagatcaacatcgggcatgtggatgccaatggactgtata
atggcagtctcaccacgtttgccctctctggattcgtccgtgcccagggt
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig15799
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||