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GrainGenes Sequence Report: BE404401

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Sequence	BE404401
Contig	Ta.9296.1.S1_at Ta.9296.2.S1_a_at NSFT03P2_Contig17804
Tracefile	[ Download ] [ View ]
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC277234
wEST map position	BE404401
NCBI UniGene	Ta.9296
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Obtusifoliol 14-alpha-demethylase (CYP51), clone w51, partial'
Keyword	EST
Germplasm	Chinese Spring
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	4AL 4BS 4DS
Clone Library	Wheat etiolated seedling root cDNA library
Tissue	Root
Developmental Stage	Five day old etiolated seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0441_G07_M13ZS Wheat etiolated seedling root cDNA library Triticum aestivum cDNA clone WHE0441_G07_M13, mRNA sequence.
Other Name	WHE0441_G07_M13ZS  [ wEST-mySQL (obsolete) ]
Strain	lab_host E. coli SOLR
DNA Library	TA008E1X
Clone	WHE0441_G07_M13
Probe	WHE0441_G07_M13
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Strategene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology	Ta.9296.1.S1_at 646 WHE2AFFY
DNA	tgtttgatgatgtttctgctctcttccatgatcttgacaatgggatgcag ccaatcagtgtcatctttccctacctcccaatccctgcacaccgtcgccg tgaccaggcacggacacgtttggcggagatcttcgccaccatcatcaagt cccgcaaggcctctggacagtccgaggaagacatgctgcagtgcttcatt gattccaagtacaagaatggccgccagaccacagaaagtgaggtgactgg gctacttatcgcagccctgtttgctggacagcacactagctcgatcacct caacctggactggggcctacctgctcaagttccagcagtactttgca

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