GrainGenes Sequence Report: NSFT03P2_Contig13288
Sequence
BE403322
Contig
Ta.7145.1.S1_at NSFT03P2_Contig13288
Tracefile
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External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC311237
wEST map position
BE403322
NCBI UniGene
Ta.7145
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001061555.1 Os08g0327100 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Germplasm
Chinese Spring
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1AL 1BL 1DL 2DL
Clone Library
Wheat etiolated seedling root cDNA library
Tissue
Root
Developmental Stage
Five day old etiolated seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0431_B06_D11ZS Wheat etiolated seedling root cDNA library Triticum aestivum cDNA clone WHE0431_B06_D11, mRNA sequence.
Other Name
WHE0431_B06_D11ZS [ wEST-mySQL (obsolete) ]
Strain
lab_host E. coli SOLR
DNA Library
TA008E1X
Clone
WHE0431_B06_D11
Probe
WHE0431_B06_D11
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Strategene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA Homology
Ta.7145.1.S1_at 515 WHE2AFFY
DNA
aggtcttcttctgagggaagatcattggaggctataacacagatacctca
gggatatcaaggtgcagggaagaaccctcaaaaagaaaacacagatggta
ggagtaaaaatatggcttcgtcggatactagagtccgggatatcaaggat
catttaatcaaggcaaaggtctaccttggtcttggagccattcgagcaaa
tcctcaatacctcanggacttgcgacatcggatacgggaagttcaaaagg
tgcttggtgaagcat
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig13288
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||