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GrainGenes Sequence Report: K02638

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Sequence
K02638
Subsequence
K02638_1.cds161119
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC168840
NCBI UniGene
Hv.66
Data at Gramene
Gramene-Protein P04747
DB Remark
Locus Source: Hordeum vulgare
UniGene title 'Alpha-amylase 1'
Keyword
alpha-amylase
amylase
Species
Hordeum vulgare
Data Source
genbankRelease 135, Apr 15 2003
Title
Barley alpha-amylase type B isozyme mRNA, complete cds, clone pHV19.
Probe
pHV19
Gene Product
alpha-amylase type B mature peptide
Remark
DB_xref: taxon:4513
Feature: source: mol_type = 'mRNA';
Locus Comment: Original source text: Barley (Hordeum vulgare L. cv. Himalaya); gibberellic acid treated aleurone cell, cDNA to mRNA, clone pHV19.; There are at least four types of alpha-amylase and there are; approximately eight copies of the gene per haploid genome. The; accumulation of alpha-amylase mRNAs in aleurones as a function of; time after addition of the hormone gibberellic acid (GA) shows; differential control of the expression of the alpha-amylase genes.; Type A isozyme mRNA is present in small amounts in unstimulated; cells, and increases in concentration upon stimulation with GA,; whereas type B isozyme mRNA is undetectable in unstimulated cells; and increases a hundred-fold after stimulation with gibberellic; acid. When excess abscisic acid (ABA) is added either in; conjunction with GA or by itself the effect of GA on alpha-amylase; is greatly diminished or no alpha-amylase is produced. The mature; peptide might start at position 85. The precise location could not; be proven experimentally.
EMBL Feature
mat_peptide881119Location: 88..>1119
sig_peptide1687alpha-amylase type B signal peptideLocation: 16..87
DNA
agagagctgaagaacatggcgaacaaacacttgtccctctccctcttcct
cgtcctccttggcctgtcggccagcttggcctccgggcaagtcctctttc
agggcttcaactgggagtcgtggaagcacaatggcgggtggtacaacttc
ctgatgggcaaggtggacgacatcgccgccgccggcatcacgcacgtctg
gctccctccggcgtcgcagtccgtcgccgagcaagggtacatgccgggcc
ggctgtacgacttggacgcctccaagtacggcaacaaggcgcagctcaag
tccctcatcggggcgctccacggcaagggcgtcaaggccatcgccgacat
cgtcatcaaccaccgcacggcggagcacaaggacggccggggcatctact
gcatcttcgagggcgtcacccccgacgcccgcctcgactgggggccccac
atgatctgccgcgacgaccggccctacgctgacggcaccggcaacccgga
caccggcgccgacttcggggccgcccccgacatcgaccacctcaacctgc
gcgtccagaaggagctcgccgagtggctcaactggctcaaggccgacatc
ggcttcgacggctggcgcttcgacttcgccaagggctactccgcggacgt
cgccaagatttacattgaccgctcggagcccagcttcgccgtggccgaga
tatggacgtcgctcgcgtacggcggggacggcaagcccaacctcaaccag
gaccagcaccggcaggagctggtgaactgggtggacaaggttggcggcaa
agggcccgctaccacgttcgacttcaccaccaagggcatcctcaacgtgg
ccgtggagggcgagctgtggcggctgcgcggcacagacggtaaggcgcca
ggcatgatcgggtggtggccagccaaggcggtgacctttgtggacaacca
cgacaccggctccacgcagcacatgtggcccttcccttctgacagggtca
tgcagggatatgcctacatcctcacgcacccagggacgccatgcatcttc
tacgatcatttcttcgactggggcctgaaggaggagatcgatcgcttggt
gtcagtcaggaccggcgcc

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