GrainGenes Sequence Report: DR741908
Sequence
DR741908
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC294235
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
EST
Species
Triticum aestivum
Clone Library
Triticum aestivum FGAS: Library 2 Gate 3
Data Source
genbank Release 145, Dec 15 2004 genbank Updated Nov 2006
Title
FGAS030949 Triticum aestivum FGAS: Library 2 Gate 3 Triticum aestivum cDNA, mRNA sequence.
Probe
MAG678 MAG1598
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Feature: source: mol_type = 'mRNA' Locus Comment: Contact: Patrick Gulick; Plant Molecular Biology; Concordia University, Department of Biology; 7141 Sherbrooke St. West, Montreal, Quebec H4B 1R6, Canada; Tel: 514 848 2424 Ext 3407; Fax: 514 848 2881; Email: pgulick@alcor.concordia.ca; This sequence is the direct result of the Base calling software; Phred (default parameters). It is the raw base calls. To aid in the; identification of the high quality insert the software Lucy; (default parameters) has been run on this sequence. Lucy identified; the region [26,769].; Plate: STG2 row: O column: 03. Note: Organ: Crown and leaf; Vector: pCMV.SPORT6; Aerial parts (crown and leaf) of wheat cultivar Norstar from control and long exposure times to low temperature. 4 mRNA populations were combined before constructing the library; 7 days non-acclimated plants and 1, 23, and 53 days cold-acclimated at 4C. Non-acclimated and cold-acclimated plants were grown in vermiculite This is the only library that was done according to the Invitrogen manual, and therefore, a percentage of clones will not have the 3 prime end because of NotI digestion within the cDNA.
DNA
tgtgtacgtgcgacccacgcgtccgcccacgcgtccgaacccagatagct
ccgtccgtgcccgattcgcttgggccgggcgtggatacctaggctaggcg
ccgccgatgtcggactgcagcacgggcacggactcgccgcgggccgactc
cgtcgacgagcagccgcccgccgacgccgagtcgcccggcggaggccaga
gcaagcgcgaccacccctcgccgtcgtcgccgctgccgccgcccaagcgc
agccggagatctgtggagaagagggtggtgtcggtgccgatttccgagtg
cggcgagcgggccaagaccaacggcgagggcccgccgccgccggactcgt
gggcgtggcgcaagtacggccagaagcccatcaagggatccccctaccca
cgggggtactaccgctgcagcagctccaaggggtgcccggcgaggaagca
ggtggagcgcagccgcgccgaccccaccgtgctgctcgtcacctactcct
acgatcacaaccacccgtggccggcgcccaagagcggctgccaacccaac
aagtcctctccgcggctggtggaccccaagcccgagcctggcacgccggt
ggagtgccagcccgagcacggacctgaagcgccggagcacgggcctgaag
cgccggagcaagatcaggaagaggagcatgagcagaagcctgtcatcggt
ctggccgaccccgcggcgatcacgacggtgaccgcggcggccgaagagga
ggaggagagcttcgacttcgggtggttcgaccagtacccgacgtggcacc
ggacggcgctatacgtgccggcgttcgacgtggcgccgcccccggaggac
tgggagcgcgagctgcagggggagggacgcctgtccgctgggctcgggga
gctcccggagtgcgccatcggtgtcggccgccggggcgagctggncttgg
gggccacgggccttgctccttacaccgggcctccggtccgatcccgtttc
cccctttcttttttcctccggctgaatttacccggagtag
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: DR741908
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||