GrainGenes Sequence Report: DR740391
Sequence
DR740391
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC293928
NCBI UniGene
Ta.23742
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_199368.1 mob1/phocein family protein [Arabidopsis thaliana]'
Keyword
EST
Species
Triticum aestivum
Clone Library
Triticum aestivum FGAS: Library 2 Gate 3
Data Source
genbank Release 145, Dec 15 2004 genbank Updated Nov 2006
Title
FGAS000338 Triticum aestivum FGAS: Library 2 Gate 3 Triticum aestivum cDNA, mRNA sequence.
Probe
MAG320
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Feature: source: mol_type = 'mRNA' Locus Comment: Contact: Patrick Gulick; Plant Molecular Biology; Concordia University, Department of Biology; 7141 Sherbrooke St. West, Montreal, Quebec H4B 1R6, Canada; Tel: 514 848 2424 Ext 3407; Fax: 514 848 2881; Email: pgulick@alcor.concordia.ca; This sequence is the direct result of the Base calling software; Phred (default parameters). It is the raw base calls. To aid in the; identification of the high quality insert the software Lucy; (default parameters) has been run on this sequence. Lucy identified; the region [15,802].; Plate: L2B0202 row: C column: 05. Note: Organ: Crown and leaf; Vector: pCMV.SPORT6; Aerial parts (crown and leaf) of wheat cultivar Norstar from control and long exposure times to low temperature. 4 mRNA populations were combined before constructing the library; 7 days non-acclimated plants and 1, 23, and 53 days cold-acclimated at 4C. Non-acclimated and cold-acclimated plants were grown in vermiculite This is the only library that was done according to the Invitrogen manual, and therefore, a percentage of clones will not have the 3 prime end because of NotI digestion within the cDNA.
DNA
cgacccacgcgtnccgtncggatcggaaggggagagcgaccggccgacca
ggaggaagcggcggcgggatgagtctcttcggcctcggacggaatcagaa
gacgttccgtcccaagaaaagtgctccatcaggcagcaagggggcacagc
tccgaaagcacatagatgcaacgctcggcagtggaaaccttagggaagct
gtgaggctgcctcctggagaggatatcaatgaatggcttgctgttaatac
tgtggatttctttaatcaagttaacctgctgtatggcacactcacagagt
tctgcacttccgagagctgcccaacaatgactgcaggcccaaagtatgag
taccgatgggctgacggtgtacagataaagaagcctatagaagtgtcagc
accaaaatacgtggagtacctaatggactggattgaaggccagcttgaca
atgaatccttatttcctcagaagcttggcacaccatttccacccaacttc
aaggatgttgtaaacacaattttcaagcgcttgtttcgtgtttatgccca
catataccattcccattttcagaagattgtcagcctcaaggaggaggctc
atcttaacacctgcttcaagcacttcattctgtttacaaacgaatttggg
ctgattgacaagaaagaactggctcccctccaggagctcatcgaatcaat
catcgttccctactgagaaggaaacactgaggctgaaccgaactaaacat
ggctgctaccgagtcctctacaggaatggtcgagcgattgggctatctgc
gccaantatttactggcacaaatcttcttgtnccatttctttgacattaa
aaaacaaaaacatgtacattaccagtgatgtatttgtgtgtacatgttca
ggagttctcctgtgcaaacgcgattgtactgctataacatgcatgttggg
gttcggtgggtatagtaataaccttgaaaagagcagttttaagatgccaa
aaaaaaaaaaaaaaaaaaaaagggcggccggcctaaagtatcctcgaggg
ggccaagcttacgcttaccagcttcctgtgaaagggggccctataggggg
cgatttaaagcaggcccgggccgcggtttaaacgccgggacggaaaacgc
gtactggggattttggagggacct

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: DR740391
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