GrainGenes Sequence Report: DR736711
Sequence
DR736711
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC277595
NCBI UniGene
Ta.41747
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, weakly similar to NP_001054262.1 Os04g0677200 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Clone Library
Triticum aestivum FGAS: Library 5 GATE 7
Data Source
genbank Release 145, Dec 15 2004 genbank Updated Nov 2006
Title
FGAS082081 Triticum aestivum FGAS: Library 5 GATE 7 Triticum aestivum cDNA, mRNA sequence.
Probe
MAG1575
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Feature: source: mol_type = 'mRNA' Locus Comment: Contact: Patrick Gulick; Plant Molecular Biology; Concordia University, Department of Biology; 7141 Sherbrooke St. West, Montreal, Quebec H4B 1R6, Canada; Tel: 514 848 2424 Ext 3407; Fax: 514 848 2881; Email: pgulick@alcor.concordia.ca; This sequence is the direct result of the Base calling software; Phred (default parameters). It is the raw base calls. To aid in the; identification of the high quality insert the software Lucy; (default parameters) has been run on this sequence. Lucy identified; the region [16,825].; Plate: L5B202 row: J column: 02. Note: Vector: pCMV.SPORT6; Crown and developmental stages of spike formation in wheat cultivar Norstar. 4 mRNA populations were combined before constructing the library. The first mRNA population is from 1cm crown sections after 30 days of cold acclimation. The second is from 1cm crown sections after 11 days of deacclimation (before deacclimation plants were fully vernalized for 49 days). The third is from different developmental stages of spike formation (5 to 50mm) that still have not emerged from the leaf (dissection required). The last is from different developmental stages of spike and seed formation after having emerged from the leaf (visible). First strand synthesis in this library was done in the presence of methylated dCTP thereby protecting from internal cleavage with NotI.
DNA
cgacccacgcttccgcccacaaccgcttctccggcgagatccctgcgggg
ataaccgcgatacggagcctgcagggcctgttccttgcggacaatcagct
gtccggggagatcccacccgggattgggaacctgacctacttgcaggcgc
tggatttgtcacataaccggctgtccggtctagtgcctgcagggcttgcc
ggctgcttccagcttctgtacctgcgtctcgggggtaatcagctctctgg
ggcgctgcggccggagcttgatgcacttgatagtctgaaggttctagatt
tgtcgaacaaccggatatctggtgagattccgctgccgctggctgggtgc
cggtctcttgaggtggtgatcttgtcgggaaatgagatcactggggagct
cagtggggctgtggcgaaatggcagagcctgaggtccctgtcgctggctg
ataatcagctctccggccagctaccagattggatgttctcattcccggtt
ctccagtggcttgatttgtctggcaataggtttgagggtttcatccctga
tggtggtttcaatgcaagttcagtgcttaatggcgcaggtggtggtcagg
gcattccatccggtggtatgatttcacctcaattgtttgtgtcagcatct
gttgattccacaggtcagcagcttgagcttggttatgatcttcgggcagc
tcctggtatagatctatcaacgaatttgctccatggagagatacctgaag
ggttggttgcaatgaagggattggagtacctgaacctctcttgcaattac
ttagctgggcagatccctgcagggcttggggggatggggaggctgcggac
gctggacttctcacataatgggctgtcaggggaggtgcctcctgtaatgc
tgccatgacagacctgaggcgcttacctctcttacataacctatctgggc
cttgccacacagatgggtacggaaatcccaggatcgtggagggacccctg
ggatatgcgtggggaaggttcatatgattcanaanncaaaaggaaaanca
gaaaa
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: DR736711
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||