GrainGenes Sequence Report: CV780471
Sequence
CV780471
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC344286
NCBI UniGene
Ta.16001
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Clone wre1n.pk0122.c2:fis, full insert mRNA sequence'
Keyword
EST
Species
Triticum aestivum
Clone Library
Triticum aestivum FGAS: Library 2 Gate 3
Data Source
genbank Release 145, Dec 15 2004 genbank Updated Nov 2006
Title
FGAS074881 Triticum aestivum FGAS: Library 2 Gate 3 Triticum aestivum cDNA, mRNA sequence.
Probe
MAG1596
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Feature: source: mol_type = 'mRNA' Locus Comment: Contact: Wm L Crosby; Bioinformatics; University of Saskatchewan, Department of Computer Science; 1C101 Engineering Building, 57 Campus Drive, Saskatoon,; Saskatchewan, S7N 5A9, Canada; Tel: 306 966 1769; Fax: 306 966 2033; Email: fgas_ests@cs.usask.ca; This sequence is the direct result of the Base calling software; Phred (default parameters). It is the raw base calls. To aid in the; identification of the high quality insert the software Lucy; (default parameters) has been run on this sequence. Lucy identified; the region [197,794].; Plate: WEF103 row: B column: 11. Note: Organ: Crown and leaf; Vector: pCMV.SPORT6; Aerial parts (crown and leaf) of wheat cultivar Norstar from control and long exposure times to low temperature. 4 mRNA populations were combined before constructing the library; 7 days non-acclimated plants and 1, 23, and 53 days cold-acclimated at 4C. Non-acclimated and cold-acclimated plants were grown in vermiculite This is the only library that was done according to the Invitrogen manual, and therefore, a percentage of clones will not have the 3 prime end because of NotI digestion within the cDNA.
DNA
cccctttttttttnnnntttttttnttnntttttttnnnnnnnnnnnngg
gctagtatggaataacagctttgacattaggcctatttaggtgactttct
aaaaaagtttgtacaaaaaagcaggccggtaccggtccggaattcccggg
atatcgtcgacccacgcgtccgagctattattatgtgtctcttaagcaaa
ggagcagtggcgtcgcaattgacagatgatggccgccttgcaagtaatat
ttgtcgaagattaacaagactaaaagattacaatgcaaagatggagcagg
gccaagagtcaaataaagataggatgtgcattgacatcctagagagggag
atgatgaggaatcctatgacagcggaagattcagtcacctcacctttatt
ggctgatgatcttcacatgaaactaagctacctggaaaatagagtcgcgt
ttgcaagattattcttccctgctgaagcgaaggttgcgatgcaaattgcg
caagcagacatcacaccagaagttggtggtttttctgcagcaagtacttc
tggtaaactgagggaagtcgatctgaatgagacgccagtaacaaaaaaca
aaaggctacgttcgagggtggatgcactagtgaaaacagtggaactgggc
cgtcggtacttcccaaactgctcgcaggtgctcgacaaattcttggaaga
tggcctgcctgatggccttgatgcattccagcagcaaagcggcacccctg
atgagcaacaggtgaagaagatgcgcttctgcgaggtgaaggaagacgtg
cgcaaagcatacagcaaagacacggcccgataacagcatgtttttcagcc
cctgtcggtcaaactccctcattcctcggccgatgaagggaaaagtacct
gtaacagggctgttttttctgg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: CV780471
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||