GrainGenes Sequence Report: CN012292
Sequence
CN012292
External Databases
Data at GenBank Data at EMBL Data at DDBJ
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
EST
Species
Triticum aestivum
Cultivar
Sumai3
Clone Library
Wheat Fusarium graminearum infected spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbank Release 141, Apr 15 2004 genbank Updated Nov 2006
Title
WHE3895_A05_A09ZS Wheat Fusarium graminearum infected spike cDNA library Triticum aestivum cDNA clone WHE3895_A05_A09, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE3895_A05_A09
Probe
MAG981
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Feature: source: mol_type = 'mRNA' Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20. No effort was taken; to identify ESTs of fungal origin from this library, thus this EST; could be of wheat or fungal origin.; Seq primer: SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes were sprayed at anthesis with Fusarium graminearum. Total RNA, and poly(A) RNA were prepared and pooled from infected spike at 0, 6, 12, 24, 36 and 48 hours after inoculation, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in G. Muehlbauer lab at the University of Minnesota (Kruger, W.M., Muehlbauer, G.J., Pritsch, C., Vance, C.). The cDNA library should contain genes of both wheat and fungal pathogen origin. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ctgcagattcggcacgagggtcgaccaatgtaagtatagcatccaactcc
accgtggccaccatcagggacagtggtagcctcgagctcattgatgcctc
caattcatcgatagtttactggcaaagcatagaccatccaacaaacacct
tgcttccaggggtaaagctcgggctgaacaaaatcactggtgttagccag
aggcttgttgcttggaagagttaggcagatccatctcctgggttgttctc
gctggagcatgacccaaacggcacaccactttatttcgcgcagtggaatg
attccataagctactgggctagtaccccttgggacttcgatctcccgggg
atggtggccttgcgattcgtcaacagctccacagaaagttacctcttgta
ctcgacgaaagacgactcggtcatcacacacatcatcatcgacattagcg
ggcagctcacgttgttgacatggctggattcctcacagcagtggatattg
atctggacccagccacataccgagtgtgatgtgtatgcgcgctgtggggc
atacggcagctgcaacctcaatgccctgccgttctgcaactgtgtcaagg
ggttcagtcagaatgactgggacct
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: CN012292
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||