GrainGenes Sequence Report: Ta.29464.1.A1_at
Sequence
CK211571
Contig
Ta.29464.1.A1_at
External Databases
Data at GenBank Data at EMBL Data at DDBJ
NCBI UniGene
Ta.29464
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, weakly similar to NP_001044123.1 Os01g0727500 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Clone Library
Triticum aestivum FGAS: Library 6 CAP GATE 1
Data Source
genbank Release 139, Dec 15 2003 genbank Updated Nov 2006
Title
FGAS023422 Triticum aestivum FGAS: Library 6 CAP GATE 1 Triticum aestivum cDNA, mRNA sequence.
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Wm L Crosby; Bioinformatics; University of Saskatchewan, Department of Computer Science; 1C101 Engineering Building, 57 Campus Drive, Saskatoon,; Saskatchewan, S7N 5A9, Canada; Tel: 306 966 1769; Fax: 306 966 2033; Email: fgas_ests@cs.usask.ca; This sequence is the direct result of the Base calling software; Phred (default parameters). It is the raw base calls. To aid in the; identification of the high quality insert the software Lucy; (default parameters) has been run on this sequence. Lucy identified; the region [43,678].; Plate: L6B002 row: K column: 18. Note: Organ: Crown and leaf; Vector: pCMV.SPORT6; Crown (50%) and leaf (50%) tissues from wheat cultivar Norstar after short exposure times to low temperature in the light and in the dark. 12 mRNA populations were combined before constructing the library. The first 6 populations: After 7 days of growth at 20Cs from wheat cultivar Norstar after short exposure times to low temperature in the light and in the dark. 12 mRNA populations were combined before constructing the library. The first 6 populations: After 7 days of growth at 20, wheat plants were transferred to 4C in the light. 1cm crown sections and green leaf tissu were separately harvested after 1, 3, and 6 hours of low temperature exposure. The last 6 populations: After 7 days of growth at 20C, wheat plants were transferred to 4C in the dark. 1cm crown sections and green leaf tissu were separately harvested after 1, 3, and 6 hours of low temperature exposure. First strand synthesis in this library was done in the presence of methylated dCTP thereby protecting from internal cleavage with NotI. In addition, this library used a primer for second strand synthesis that annealed to an artificial sequence (RNA oligo) added before first strand synthesis. Therefore when sequences from EST generated from this library will be masked for vector and adaptor sequences, an additional masking step will have to be included to mask this RNA oligo that is common to all clones (sequence CGACTGGAGCACGAGGACACTGACATGGACTGAAGGAGTAGAAA).
DNA
gccctttttttttttttttttttttttttttttttttttttttttgcagg
taatattttcatgaaaaggaataaaaaaaaatgatctaaatgtattttag
ttctaaatacatcgttttttgttcattttgataacaagtattttcggacg
gagggagtagtacagtcgtaaattattcagaaaaatgtatactattccat
tccaaaaaaaaatcctaactcactactggccgatttgcatcaaccaacgg
atgcaaattaattaattgggtgggcaaccacggggaaaatgagccgaaca
aaattgaccggccaacaccgtcgggaaaacgcgtggtgactgacttttct
cgcacatatgcacagatgacgggtccgcctcgtccgtcacccttcgatga
agccggtcatccgcagcacggagttgcgcaggtggttgaggtcccggtgc
gcgcgcccgggcccggaccgcagcgaaaaggccgccgccgccttcccctc
cgaccacctgggcgacaccatcaggtgcggcggcaccatggggccgtcct
cctcctcctcctcgtcgtcgtccaggccagcgcgcccgccggacaggtgc
gcggccctgggaatgtccaccggcgcagagcactgccggggggccctgga
ggccctgggagccgccgtcaatggcccccacgcgctccccggagcgccgt
ggcgcgtctccggccacagcacctccgcatcgggacagttcctcctccac
cgtcgacatgtctgtgcgggttttgtacttcccccacgtatgcacccggt
aattgagtgaccaccgggattggaaaccccaagaatcttccttttcgggt
ttttcctaccctttaacccaggtcaaggttctccgggcccaattcgggac
cggtgggttaaaaatttccgggaatccggaccggtaccccctggtttttt
gtaaaacttgtctttagtgtccctaaaaggcctagggtcatactgttccg
ggttaaattttattccccccgggcccatagaaaactggagggcggatccc
ccccggttttttatgggggcaacaacggggatgggctcccgggcatttgg
ggtaaaaagagccttggtttttaacccccccgccc

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: Ta.29464.1.A1_at
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