GrainGenes Sequence Report: CK207680
Sequence
CK207680
Contig
TaAffx.49246.1.A1_at
External Databases
Data at GenBank Data at EMBL Data at DDBJ
NCBI UniGene
Ta.37551
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001047223.1 Os02g0578100 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Clone Library
Triticum aestivum FGAS: Library 5 GATE 7
Data Source
genbank Release 139, Dec 15 2003 genbank Updated Nov 2006
Title
FGAS019331 Triticum aestivum FGAS: Library 5 GATE 7 Triticum aestivum cDNA, mRNA sequence.
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Wm L Crosby; Bioinformatics; University of Saskatchewan, Department of Computer Science; 1C101 Engineering Building, 57 Campus Drive, Saskatoon,; Saskatchewan, S7N 5A9, Canada; Tel: 306 966 1769; Fax: 306 966 2033; Email: fgas_ests@cs.usask.ca; This sequence is the direct result of the Base calling software; Phred (default parameters). It is the raw base calls. To aid in the; identification of the high quality insert the software Lucy; (default parameters) has been run on this sequence. Lucy identified; the region [19,804].; Plate: L5B011 row: J column: 23. Note: Vector: pCMV.SPORT6; Crown and developmental stages of spike formation in wheat cultivar Norstar. 4 mRNA populations were combined before constructing the library. The first mRNA population is from 1cm crown sections after 30 days of cold acclimation. The second is from 1cm crown sections after 11 days of deacclimation (before deacclimation plants were fully vernalized for 49 days). The third is from different developmental stages of spike formation (5 to 50mm) that still have not emerged from the leaf (dissection required). The last is from different developmental stages of spike and seed formation after having emerged from the leaf (visible). First strand synthesis in this library was done in the presence of methylated dCTP thereby protecting from internal cleavage with NotI.
DNA
aacgagagcggccgccctttttttttttttttttttttaagagcaggtcc
aacccgtaccatttttgaactctaaatgcctaaaaatcaaaggcgaggga
attagatgtgagagggagttagatgtgagataatatctcacatctagatg
tgacatagacagacccaaaattgatatcctgtttgcacggccgcgggaga
aggacgatccaacggtggtgcttgtatattcttgaacagaacctcaaggt
tggcgaacgatctgccacaaggttgcgtcgtcgcacggacggcgatctcc
ttccactccatcgccttccgcctcatctccttccccacctcgtcagcacc
catcaccatccttatcctctcctcgaccacctcccggcgcacgtcgtcgc
cgacctccatcccaacgccccactccacgcacgcgtagcggcagttggtc
tgctgctcggcgaagaagggccagcccagcatcggcagcccggcgctgag
cccctccatcatggagttccacccgtagtgcgtcaggaagatgcccacgg
cctcgtgccgcagcaccgcctcctgctcgcaccagctccccaagaaacac
ctgcccttggtcgcctctacgaagtccgggggcagcactgcggcgtcacc
cttcaccaggtcgttcctcacgatccagaggaagtcgtagccgcagttgg
ccagcccccaggcgaactccaccagctcaaggctggtcatggtggtgatg
ctccccgaagtgacgtacaccacggactggggctccctgccgcggagcca
ctctaggcaggaccggtcttccttccaaggctggggcgaatcgcagcgag
ggggacgctgccatcgccgccggcggggactacctgctccaaaccaaagt
tgagcggggccattgtgtaaaactggcggggtaaggctgcccccaatggc
tccaatgccggtttgccccaactccttaaaagggtttgaccaataatggg
ttccgaagtgttcgcccgccctaacccgggttttattaaattgaacaggg
gtttccccgggttcccccttggggaataaaggagg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: CK207680
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||