GrainGenes Sequence Report: Ta.25903.1.A1

Locus Comment: Contact: Wm L Crosby; Bioinformatics; University of Saskatchewan, Department of Computer Science; 1C101 Engineering Building, 57 Campus Drive, Saskatoon,; Saskatchewan, S7N 5A9, Canada; Tel: 306 966 1769; Fax: 306 966 2033; Email: fgas_ests@cs.usask.ca; This sequence is the direct result of the Base calling software; Phred (default parameters). It is the raw base calls. To aid in the; identification of the high quality insert the software Lucy; (default parameters) has been run on this sequence. Lucy identified; the region [9,767].; Plate: L5B004 row: M column: 17.

Note: Vector: pCMV.SPORT6; Crown and developmental stages of spike formation in wheat cultivar Norstar. 4 mRNA populations were combined before constructing the library. The first mRNA population is from 1cm crown sections after 30 days of cold acclimation. The second is from 1cm crown sections after 11 days of deacclimation (before deacclimation plants were fully vernalized for 49 days). The third is from different developmental stages of spike formation (5 to 50mm) that still have not emerged from the leaf (dissection required). The last is from different developmental stages of spike and seed formation after having emerged from the leaf (visible). First strand synthesis in this library was done in the presence of methylated dCTP thereby protecting from internal cleavage with NotI.

DNA

gccgcccttttttttttttttttccaggtgagccaaattcgactacaaaa
tacatggaccaaaaggggtgcggtattactgtatcaagtctgcgtatgtg
aatcatgttccactatacatcaagaggccagcacaatgaaactgaacctg
aagtctatgcaaagcacgatccgtcggtgaagtccagacactggaagttc
gtcaggcaggatgaccggtttgtgctgctctcttctgcaagtactgttgc
tcctatcttcagctgtctcgagcctgtagaagaaccccggacttaggttg
cctcattgtcccagtagttcctcaactgcgacatatggaggagcagtccg
tcacggcctagtcctgtggtgctgctcgtcatgatccatggaggaggctt
tggatagagcgagccaatgttctccaggaacactttgatgttctcatcag
gccgaccccctttgcctttctttactttgtcacacttggtgaacacaaag
gttagcccgacattgttacgaccaagccagttagcacagtcaaggtcaat
ttgttgagttggaacactagcatcaacaaggagcagtacaccgaccaaag
tgtccctgttcaagaaataccccttggtaaatgaagaccaatccgttcga
gctgtcttggacgcagctgccaacccgtaaccaggcaaatccacaaggta
ccaacttttgttaatcaaatgatggttaattgtttgagtcttcccaggct
tcttggaggtgagtgcgacttccttgcggcgcgtgagtgtattgatgaga
gaggacttgccgacgttggagcggccgaggacggcgaactcagggcgggc
gtccttcgggcaattcccgggcgttcgccctgcacccgatgaactcagcc
tcttttacctcgccgtcccggggtacggtcccaccaagatgttcgacccc
ggaaattaaatctccgccgggaaaagcggggtccgggttatccccggggg
ggaaaaaaaggcccgttccattgggcccctaagggggctcggcctcccgg
aaattcttggggcgggttcggc


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