GrainGenes Sequence Report: CK171686

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Sequence

CK171686

Contig

TaAffx.99763.1.S1_at

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Triticum aestivum (bread wheat)

Keyword

EST

Species

Triticum aestivum

Cultivar

Wheat line CI 14106

Clone Library

Triticum aestivum FGAS: TaLt6

Data Source

genbank	Release 139, Dec 15 2003
genbank	Updated Nov 2006

Title

FGAS047053 Triticum aestivum FGAS: TaLt6 Triticum aestivum cDNA, mRNA sequence.

Strain

lab_host DH5 alpha

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Wm L Crosby; Bioinformatics; University of Saskatchewan, Department of Computer Science; 1C101 Engineering Building, 57 Campus Drive, Saskatoon,; Saskatchewan, S7N 5A9, Canada; Tel: 306 966 1769; Fax: 306 966 2033; Email: fgas_ests@cs.usask.ca; This sequence is the direct result of the Base calling software; Phred (default parameters). It is the raw base calls. To aid in the; identification of the high quality insert the software Lucy; (default parameters) has been run on this sequence. Lucy identified; the region [61,896].; Plate: TaLt614 row: K column: 04.

Note: Organ: Crown; Vector: pGEM-T; SSH (suppression subtractive hybridization) cDNA library from genotype CI14106 cold hardened at 2 C for 1 day (24 H)(tester) and subtracted against genotype CI14106 non-hardened (20 C) (driver). Nitro-pyrole anchored oligo-dT priming and non-directional cloning.

DNA

cttccaacgcgttcgggagctctccctnatggtcgacctgcaggcggccg
cactagtgattctaatacgactcactatagggcagccggccgaggtccgg
caactgggccttctccaccgcccgcctcaatctccacctcgccctcctcg
ccggggaaagaggaggatgcataatagttgattcaacaaggaaagggaaa
cgatttccagatagcatggcaaagacaatacccatttggtgctgcgttct
caatcgagccattcagaggcatcgactgcgggctatcaaccaaagtagcg
gattgaattccgaaatgtcagctgttgtatcaaacggggatgctgaagag
tactctggttcatcaaactgggatagctcagttcatcttcctgtatgggt
tctggaaactgagagaaatgcaatagaggggcgtattgaagaatgggcgg
acctatttgaatcttgtggtgcagacattcattctcttgcattaggtttg
caaaaaccactccgtccactgtggatatcacaaaatacacgtatatggtt
gaatgaagtaccagatcatcagttatgggacttcactcccatcatattag
tttcagcatctgcatctggtacagtggctacacaaagaacggcatcagaa
ttcagctggcgctatattcctggtgcaggagatgatgaagagagttgggc
acgtggtctaactcctacattattctggaagcattcgtatgatctacttg
atggtggaccagatctttgtaatcagttagttgccgacatcgttgaaaag
gatagggtttaccgtgcacagaggggtgaacattctccacaagttatagt
ttagcatataaagtgctcaagctatggancagaatcatacacgggagatc
atacanttattacacaaactatgaactcgaacccttctactcttgctccc
agcaatacacattactctaatgatggtcatgtaagtctctggattgggga
atccaacattggagnagcattctaccagccaaaagggcttggctgaattt
gaattggattcggaactggggaaacccacttaaaattgccctcttatttc
tataaaaaaatcttcccttgtaacacccttttgggagttccggggataaa
agaattttttgggaaaaaattttcccaaaagccttggctttggaaaaaaa
aaaccatctccgggggaaaaaga


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