GrainGenes Sequence Report: CK162192

Locus Comment: Contact: Wm L Crosby; Bioinformatics; University of Saskatchewan, Department of Computer Science; 1C101 Engineering Building, 57 Campus Drive, Saskatoon,; Saskatchewan, S7N 5A9, Canada; Tel: 306 966 1769; Fax: 306 966 2033; Email: fgas_ests@cs.usask.ca; This sequence is the direct result of the Base calling software; Phred (default parameters). It is the raw base calls. To aid in the; identification of the high quality insert the software Lucy; (default parameters) has been run on this sequence. Lucy identified; the region [33,747].; Plate: L4B004 row: D column: 01.

Note: Organ: Crown and leaf; Vector: pCMV.SPORT6; Aerial parts (crown and leaf) of wheat cultivar Norstar during dehydration stress. 8 mRNA populations were combined before constructing the library. The first four come from removing plants from vermiculite (7 day old plants) and incubating them at 20C on the bench without water for 1, 2, 3 and 4 days. The last four come from plants grown in soil in a growth chamber after watering is terminated. Four samplings were taken in a two week period; the first after wilting was observed and the last, two weeks later, consisted of live crown and stem tissue (leaf tissue was yellow and dead). First strand synthesis in this library was done in the presence of methylated dCTP thereby protecting from internal cleavage with NotI.

Note: Organ: Crown and leaf; Vector: pCMV.SPORT6; Conditions for growth: Seeds were germinated in a water-saturated mix (50% black earth and 50% ProMix) in a growth chamber for 7 days under an irradiance of 200 mmol m-2 sec-1. The temperature was maintained at 20 degrees C with a 15-hr photoperiod under a relative humidity of 70%. After this period watering of plants was stopped. Four time points were sampled during a two week period; the first after wilting was observed and the last, two weeks later, consisted of live crown and leaf tissue (leaf tissue that was yellow was not included in sampled material).First strand synthesis in this library was done in the presence of methylated dCTP thereby protecting from internal cleavage with NotI.

DNA

cgcttccgggaacaggaagaatttcatagatagggggacagcgagagaca
aacgcaacgagatgtcagcggagactgagaaaagcgccgacgggccggcg
cccgcgcccgcgcccatgcggtgccagcgaataggctgtgacgccatgta
caccgacgacgacaaccccgacggctcctgccactaccacccctccggac
ctatgtttcatgatggcatgaaagagtgcagctgttgcaagcaaagaagc
catgattgtagcttattttaggctattcctggcatgtgccacagggaagc
atacaactgagaaaccagtcacaaaagctgtttctcttaactcaaaggca
accccaccaaagttagctccaatcctgtcttctaaacatggtgtggaaac
cgaggcctgctccaggtgccgacagggtttcttttgctcctaccatggat
cacagcccaaggcacaaaaaccagttgctgtaaatggtacaaatacagaa
cctgtcgaaaaatgctcagttccacagcccaagaaaaaagttgttaatat
aagtgagcctagggtttgtaagaataaaggatgtggtagaacctacatag
agaaagataaccatgatgctgcatgtgaataccatccaggtcctgcggtt
ttccatgacaggaatacagggtggaagtgtagcgatgtccatgtcaagga
gtttgacgaattaatggagatacctccatgcgcaaagggctggcacaatg
ctgatgccgtatgagttccaccatgactctcgcattgagagttatatgag
ttgctatatctcctggtgcatatggtaggcatctgcccgtatttctggac
aactgtgcttccactgtgacctgataccgagaatacgctgcttgagtgag
tcattactcgaaaacccatgtgacgtttcaagtatttggaatcggacctg
aaaacctatgtggcgcccaatttattatttactatgcccttttggggtnn
aaanccntncaacgcggttcccggtcttggtggggagaagttttcaacct
cctccaaaaaaaaaggggggggctctaatatcctcgggggccctctttct
cccaagttgtgggggtgggcttattta


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