GrainGenes Sequence Report: CK157683
Sequence
CK157683
External Databases
Data at GenBank Data at EMBL Data at DDBJ
NCBI UniGene
Ta.54659
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001046448.1 Os02g0252100 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Wheat line PI 178383
Clone Library
Triticum aestivum FGAS: TaLt4
Data Source
genbank Release 139, Dec 15 2003 genbank Updated Nov 2006
Title
FGAS038835 Triticum aestivum FGAS: TaLt4 Triticum aestivum cDNA, mRNA sequence.
Strain
lab_host DH5 alpha
Probe
MAG2131
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Wm L Crosby; Bioinformatics; University of Saskatchewan, Department of Computer Science; 1C101 Engineering Building, 57 Campus Drive, Saskatoon,; Saskatchewan, S7N 5A9, Canada; Tel: 306 966 1769; Fax: 306 966 2033; Email: fgas_ests@cs.usask.ca; This sequence is the direct result of the Base calling software; Phred (default parameters). It is the raw base calls. To aid in the; identification of the high quality insert the software Lucy; (default parameters) has been run on this sequence. Lucy identified; the region [160,413].; Plate: TaLt418 row: G column: 03. Note: Organ: Crown; Vector: pGEM-T; SSH (suppression subtractive hybridization) cDNA library from genotype PI178383 cold hardened at 2 C for 1 day (24 H)(tester) and subtracted against genotype Norstar cold hardened at 2 C for 21 days and 49 days (equal amount of cDNA pooled together before subtraction, driver). Modified Smart cDNA (Clontech) priming and non-directional cloning.
DNA
naaccgctacctagagtgattacgccaacctatttagtggacactataga
atactcaagctatgcatccaacgcgttgggagctctcccatatggtcgac
ctgcaggcggccgcactagtgattagcgtggtcgcggccgaggtgggcgg
ganaancnactgagaaattggttacattctctgcggtcgccgcatctcca
tctagctacttccgctccattggttcgagtaaaacctttcgaggtgtgtt
gtattgcttgttagcggtcacagacgttatgttcccgcttgtggctgttg
aatcttaaggcctgtancatggcgtgtcagtcatatctatgtaccatgtg
tgtggattgtcagggacccattttccatgtactttgtttgggaaaaagct
tgtgagttgatccagcaaanaanaaaacaccaaaaaaaaacccaccccag
agggcagcggcgcacgcccgcagaaaactcgcaaaaaaaaaaggcccacc
ttcccgcgggggcccttgaaaaatcccccggccatgcgggccgggaactt
tcaatttggggcccaattcccgctatggtgggtcgatatacaaatccccg
gcccggcgttttacaacttccgacatgggaaaaacccggggttacccaaa
ataatccgccttggcaaaaaatccccttttggcacaccccgcgttaattc
ccaaaaaggccgcaccaatatcccctcgacaaacggtgggccgcacagaa
acgccaaaagggcccgccccggaaaaccggccattaccccgcccgaggga
tgctgaaaccaccccagctgcccccaaaaccttcgcccgcgcaatcgccc
gcgcccttccgcgattctccgaaagcttcctctcgcgccggcggagctgt
tccccgttccagaatcattttttgtg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: CK157683
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||