GrainGenes Sequence Report: CK152581

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Sequence

CK152581

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Transcribed locus, moderately similar to NP_001131798.1 hypothetical protein LOC100193171 [Zea mays]'

Keyword

EST

Species

Triticum aestivum

Cultivar

Wheat line CI 14106

Clone Library

Triticum aestivum FGAS: TaLt3

Data Source

genbank	Release 139, Dec 15 2003
genbank	Updated Nov 2006

Title

FGAS035653 Triticum aestivum FGAS: TaLt3 Triticum aestivum cDNA, mRNA sequence.

Strain

lab_host DH5 alpha

Probe

MAG1881

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Wm L Crosby; Bioinformatics; University of Saskatchewan, Department of Computer Science; 1C101 Engineering Building, 57 Campus Drive, Saskatoon,; Saskatchewan, S7N 5A9, Canada; Tel: 306 966 1769; Fax: 306 966 2033; Email: fgas_ests@cs.usask.ca; This sequence is the direct result of the Base calling software; Phred (default parameters). It is the raw base calls. To aid in the; identification of the high quality insert the software Lucy; (default parameters) has been run on this sequence. Lucy identified; the region [124,808].; Plate: TaLt349 row: L column: 18.

Note: Organ: Crown; Vector: pGEM-T; SSH (suppression subtractive hybridization) cDNA library from genotype CI14106 cold hardened at 2 C for 21 days and 49 days (equal amount of cDNA pooled together before subtraction, tester) and subtracted against genotype Norstar cold hardened at 2 C for 1 day (24 H)(driver). Nitro-pyrole anchored oligo-dT priming and non-directional cloning.

DNA

taccagctattgcgattgattacgccacgctatttatgtgacactataga
atactcaagctatgcatccaacgcgttgggagctctcccatatggtcgac
ctgcaggcggccgcactagtgattcgcggccgaggtgccatttcagagat
aaattgggccaagggtgctacggctctgtgtacaagggcgtgctactccc
aggcggcgtccatgtcgccgtcaagatgctagagggcaattccaactgca
atggagaagatttcatcagcgaggtctccaccatcggcaggatccaccac
gtcaacgtggtgcgtttagtggggttctgctcagtggaaatgcgaagggc
cttagtctacgagtacatgcccaatggttctctggacaagtacatcttct
ctaccgagaagagcttctcatgggacaagctcaacgagatcgctttgggc
attgccagggggatcaactacttacatcaggggtgcgacatgtagattct
gcactttgacatcaagccgcacaacatccttcttgacagcaatttcatcc
caaaggtcgccgattttgggctcgccaagctgtaccctaagggagacagt
ttcgtgccactgagtgccatgcggggaaccataggctacatagcccccga
gatgatatcccggagcttcggcgtcatatctagcaagtccgacgtgtaca
gcttcgggatgttgctgctggagatggccggcgggagaaggaacgctgat
ccaaagatggggtccctcagccaggcgtactacccatcatgggtgtatga
ccagctgactcaagaagaagcgggcgagatatctccggttgctgcccaca
tgcacgagctggagaagaagctgtt


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