GrainGenes Sequence Report: CF133001

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Sequence

CF133001

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Triticum aestivum (bread wheat)

Keyword

EST

Species

Triticum aestivum

Cultivar

Chinese Spring

Clone Library

Wheat meiotic floret cDNA library

Tissue

Whole florets with anthers

Developmental Stage

Meiotic stages pre-meiosis-metaphase I

Data Source

genbank	Release 137, Aug 15 2003
genbank	Updated Nov 2006

Title

WHE4352_H03_O06ZT Wheat meiotic floret cDNA library Triticum aestivum cDNA clone WHE4352_H03_O06, mRNA sequence.

Strain

lab_host E. coli DH10B

Clone

WHE4352_H03_O06

Probe

MAG681

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: T7 primer.

Note: Vector: pSPORT1; Site_1: SalI; Site_2: NotI; Plants were grown in a glasshouse. Anther meiotic stage was determined microscopically after removing a single anther from a primary floret. If determined to be between (and including) meiotic stages pre-meiosis and metaphase I, remaining floret tissue was collected and pooled for library construction. The tissue, total RNA, and poly(A) RNA were prepared, cDNA synthesised, and directionally ligated into pSPORT1 by Tim Sutton in the P Langridge Lab at the Department of Plant Science, University of Adelaide, Waite Campus, Australia. Average insert size 1.4Kb. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

ccggtccggattcccgggtcgacccacgcgtccgtgaagaagtccaaaca
tttgcgtgttcttgatctcagtggctataactccaaagagttacctcatt
gcatttatgacttactgcacctccgttacctatctatccatggctccatc
cagagacttcctgagtcgattggcaagcttctgcatctgcaaacattgtg
catctctgggaaatgttcccttgagaagcttcctgcgagcgttagtatgt
tggtcaatttgcggcatcttatggttgaaatgaagtatactgcaggactg
gtaggcattggccggctagcaaaccttcagggatcacttgagctccacat
tgcgaagttggaagggcgcaaaccagaagaattgaggaacattgatggtc
ttcatgggttagtaataaaatgtctagaaaatgtttcaggctatgaagaa
gcccgcaaagctgagttgaataagaaactttatcttaattttctgaattt
ggaatggagctcagctagtagaaatagctcccctcctgctgatgcaaagg
tgcttgaaggtctgcggcctcaccatgccataaaggtacttaatattaga
aggtattgtggcgccaaagctcctagttggctgcagtcactgcagcaact
acgttctttgcgcctcatcaattgcaggagcttgtgcatccttccctcat
tggg


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