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GrainGenes Sequence Report: CF132900

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Sequence	CF132900
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC280752
NCBI UniGene	Ta.58245
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001131654.1 hypothetical protein LOC100193014 [Zea mays]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Clone Library	Wheat meiotic floret cDNA library
Tissue	Whole florets with anthers
Developmental Stage	Meiotic stages pre-meiosis-metaphase I
Data Source	genbank Release 137, Aug 15 2003 genbank Updated Nov 2006
Title	WHE4351_F05_K09ZT Wheat meiotic floret cDNA library Triticum aestivum cDNA clone WHE4351_F05_K09, mRNA sequence.
Strain	lab_host E. coli DH10B
Clone	WHE4351_F05_K09
Probe	MAG1874
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: T7 primer. Note: Vector: pSPORT1; Site_1: SalI; Site_2: NotI; Plants were grown in a glasshouse. Anther meiotic stage was determined microscopically after removing a single anther from a primary floret. If determined to be between (and including) meiotic stages pre-meiosis and metaphase I, remaining floret tissue was collected and pooled for library construction. The tissue, total RNA, and poly(A) RNA were prepared, cDNA synthesised, and directionally ligated into pSPORT1 by Tim Sutton in the P Langridge Lab at the Department of Plant Science, University of Adelaide, Waite Campus, Australia. Average insert size 1.4Kb. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	tggacgcctgcaggtaccggtccggaattcccgggtcgacccacgcgtcc gctaaaaccttaaaccctagctccggtcacccccccatggcggccgtccg ggtcatcctccctcatccccacctccccatcccctcgtggagaccccagc tcaagccctcctcatcctatccccgccgcgcggtcgccgccccagcttcg ctgtctccgtcgcccctcacggcttccctgcaactcggagatgtgatcga ggcgcagcagttcgatcggcaggtgcttaatgagatctttgaggtggcgc gggagatggaggccgtggagcgcggctcccacgggttccggagccgcgtc cttgaggggtacctcatggccacgctcttctacgagccctccactcgcac gcgcctctccttcgaggccgccatgcgccgtcttggcggagaggtgctca ccaccgagaacgctcgcgagttctcgtccgctgccaagggcgagacccta gaagataccataagaaccgttgaaggttattctgatattatcgttctgag acattttgaaagtggagctgccagaagagcagcggctactgcagaaattc cggttattaatgcaggtgatgggccagggcaacatcctactcaggctctg ttggatgtatatacaataaagagagaaattggtaggctagatggaatcaa acttggtttggttggagaccttgccaatgggagaactgttcgttctctgg cctatttgatcgctaagtaccagaacattaagatatacttcgtatctcca g

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